| 牛IL-18基因的克隆及遗传进化分析 |
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| 引用本文： | 张林,金宁一,马鸣潇,李昌,金明兰,郑敏,金扩世,夏志平.牛IL-18基因的克隆及遗传进化分析[J].中国免疫学杂志,2006,22(1):68-71. |
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| 作者姓名： | 张林 金宁一 马鸣潇 李昌 金明兰 郑敏 金扩世 夏志平 |
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| 作者单位： | 1. 中国人民解放军军事医学科学院,全军重点基因工程实验室,长春,130062
2. 锦州医学院畜牧兽医学院,锦州,121001 |
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| 基金项目： | 国家高技术研究发展计划(863计划);吉林省科技厅科技发展计划 |
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| 摘 要： | 目的：克隆牛白细胞介素18（IL-18）全基因，并对其进行序列分析。方法：从ConA刺激培养的牛外周血淋巴细胞提取总RNA，利用巢式RT-PCR方法扩增出牛IL-18全长cDNA，将其克隆到pMDl8-T载体上，测序后进行序列分析。结果：成功地克隆到了牛IL-18全基因，序列分析表明，实验中所克隆到的牛IL-18序列与GeneBank所登录的牛IL-18核苷酸序列及其推导氨酸序列同源性分别是99．5％和99％。与人、猕猴、野猪、山羊属、马等核苷酸序列及其推导氨基酸序列同源性分别在84．9％-99．5％和74．9％～99％之间，研究结果在国内还未见报道。结论：成功地从牛外周血中克隆到了牛IL-18的基因，其全长为598bp。
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| 关 键 词： | 牛白细胞介素18 克隆 序列分析 |
| 文章编号： | 1000-484X（2006）01-0068-04 |
| 收稿时间： | 2005-06-30 |
| 修稿时间： | 2005-06-302005-08-16 |
Cloning and sequence analysis of bovine interleukin-18 |
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| ZHANG Lin,JIN Ning-Yi,MA Ming-Xiao,LI Chang,JIN Ming-Lan,ZHENG Min,JIN Kuo-Shi,XIA Zhi-Ping.Cloning and sequence analysis of bovine interleukin-18[J].Chinese Journal of Immunology,2006,22(1):68-71. |
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| Authors: | ZHANG Lin JIN Ning-Yi MA Ming-Xiao LI Chang JIN Ming-Lan ZHENG Min JIN Kuo-Shi XIA Zhi-Ping |
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| Institution: | Institute of Military Medical Sciences of PLA, The Key Laboratoy of Genetic Engineering, Changchun 130062, China |
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| Abstract: | Objective:To clone a cDNA of bovine interleukin-18 and analyze sequence. Methods:The full length cDNA of boIL-18 from the bovine peripheral blood lymphocyte which is stimulated by ConA was amplified by nest-PCR. It was cloned in pMD18-T vector and sequence was analyzed. Results: Homology between the cloning sequence and the sequences of boIL-18 registered in the genbank are 99. 5% and 99%. Homology of nucleotide sequence between the cloning sequence and the sequences of Homo, Macaca, Sus scrofa, Capra and Equus is in 84.9% -99.5%. Homology of amino acid sequence of them is in 74.9% - 99%. Conclusion: BoIL-18 from the bovine peripheral blood lymphocyte has been cloned successfully and its full length is 598 bp. |
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| Keywords: | Bovine interleukin-18 Cloning Sequence analysis |
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