供者源性的骨髓间质干细胞对急性移植物抗宿主病的影响

目的:采用大鼠骨髓移植模型探讨共移植供者源性的骨髓间质干细胞(MSCs)对骨髓移植后急性移植物抗宿主病(GVHD)的影响。方法:体外培养Fisher344大鼠骨髓间质干细胞,扩增至第5代用于移植。建立大鼠异基因急性GVHD模型(F344→Wistar)。受者Wistar大鼠采用致死性全身照射预处理,当天移植F344大鼠骨髓细胞和脾细胞。实验组则移植F344大鼠骨髓细胞、脾细胞和第5代的MSCs。观察各组移植后急性GVHD的发生时间、发病率和存活时间。结果:共移植MSCs推迟急性GVHD的发病时间,延长该组的存活时间,但无法完全消除急性GVHD的发生。结论:MSCs在体内具有免疫抑制功能,供者来源的MSCs在不使用免疫抑制剂情况下,可推迟急性GVHD的发病时间和延长受者的存活时间。

Effects of cotransplantation of donor-derived bone marrow mesenchymal stem cells on acute graft versus host disease

AIM:To study the effects of cotransplantation of donor-derived bone marrow mesenchymal stem cells on graft versus host disease in a rat allogeneic bone marrow transplantation model.METHODS:Fisher 344 rat bone marrow MSCs were isolated and cultured to the fifth passage (P5) in vitro. The recipient Wistar rats were conditioned with lethal total body irradiation and transplanted with F344 rat bone marrow cells and spleen cells in the presence or absence of (P5) MSCs. The onset time of graft versus host disease (GVHD), incidence of GVHD and survival time were monitored.RESULTS:Cotransplantation of MSCs deferred the onset time of GVHD and prolonged the survival time, but did not block GVHD.CONCLUSION:MSCs have immunosuppresive activity in vivo. Donor-derived MSCs delay the onset of GVHD and prolong survival in the absence of immunosuppressive agent.