两种计数儿童网织红细胞方法比较

目的比较XE-2100全自动血液分析仪和人工显微镜法测定儿童网织红细胞的优势。方法对343例病人标本的检测结果进行统计学处理,比较观察。结果两种计数儿童网织红细胞方法无论高、中、低值均无显著性差异,相关性良好,r=0.992。而XE-2100血液分析仪计数高、中、低值网织红细胞的重复性均优于人工显微镜目测计数。结论XE-2100血液分析仪检测RET具有方便、快捷、精密度高、准确性好的、检测参数多、干扰少等优点,能更好地提供相关病情诊断及治疗转归信息;显微镜目测法计数网织红细胞作为一种能直观细胞形态的传统方法应与全自动分析仪相互结合使用,可使实验结果更加准确、科学。

COMPARISON OF TWO METHODS OF CHILDRENS RETICULOCYTE COUNTING

Objective To compare two methods of children’s reticulocyte counting, namely, XE-2100 automated hematology analyzer detection and microscopic examination. Method Reticulocyte of venous blood samples collected from 343 children patients were counted by these two methods respectively. Statistical results were obtained by using the SPSS 11.0 software. Result The two methods used in the present study had no significant difference statistically, no matter what level of counting was measured. Correlation coefficient of these two methods was 0.992 as indicated that the correlation was fine. Repeated trials resulted in a lower CV value in XE-2100 detection than in microscopic examination which meant the former had a better reproducibility. Conclusion Reticulocyte counting performed by XE-2100 automated hematology analyzer was more convenient, accurate, precise and faster than by microscopic examination. Moreover,the XE-2100 could output multi-itemed, automatic and stable results so as to provide more detailed information for the treatment. As a traditional detection method, the microscopic examination combined with the XE-2100 could provide direct morphological and more accurate results.