人胃癌顺铂耐药细胞系的建立过程

目的:建立人胃癌顺铂耐药细胞系方法:采用逐步递增顺铂浓度,间歇作用体外诱导法建立人胃癌顺铂耐药细胞系SGC7901/ DDP,每月作相关检测;MTT法测定药物敏感性:光镜、电镜、MTT法计数活细胞、流式细胞仪等方法观察其生物学特征的改变.结果:历时4 mo建成人胃癌顺铂耐药细胞系SGC7901/DD P,1,2,3,4mo的耐药指数分别为2.13,5.32,12.60,12.93,并且与5-氟尿嘧啶、丝裂霉素等多种抗癌药有不同程度的交叉耐药性:SGC7901/DDP的细胞形态逐渐发生改变;体外群体倍增时间较亲代细胞逐渐延长:细胞周期分析发现其S期与G_2/M期细胞逐渐减少,G_0/G_1期细胞逐渐增多.结论:SGC7901/DDP细胞具有耐药表型特征,耐药性能稳定,增殖能力逐渐下降.

Construction of a cisplatin-induced human gastric cancer drug-resistant cell line

AIM:To establish a cisplatin-induced human gastric cancer drug-resistant cell line and study changes in its resistance. METHODS:A resistant gastric cancer cell line (SGC7901/DDP)was established by gradually increasing the dose of cisplatin with intermit- tent,monthly administration.Drug sensitivity was detected by MTT assay.Changes in biologi- cal characteristics were determined using light microscopy,electron microscopy,cell counting by MTT assay,and flow cytometry. RESULTS:The SGC7901/DDP cell line was developed after 4 mo,with stable resistance to cisplatin.The resistance index for each month was 2.13,5.32,12.60 and 12.93,respectively. SGC7901/DDP cells exhibited cross-resistance to many other chemotherapeutic agents(e.g., 5-fuorouracil and mitomycin C).The morphol- ogy of SGC7901/DDP cells gradually changed; doubling time was gradually prolonged,and the cell numbers in S- and G_2/M-phases gradually decreased,while that in G_0/G_1 phase gradually increased,compared with parent cells. CONCLUSION:The SGC7901/DDP cell line showed a typical and stable resistant phenotype, multiplication of the SGC7901/DDP cell line gradually decreased,and MTT assay and elec- tron microscopy determined the resistance of the SGC7901/DDP cell line.