| 摘 要： | Results of the establishment and application of anti-interferon antibodycolumn are reported.Antiserum against human lymphoblastoid interferon(HuLyIFN)was obtained by immunizing sheep with partially purified Namalvainterferon.After they were isolated from antiserum,the immunoglobulins againstHuLyIFN were adsorbed repeatedly on the“mock interferon”column and trypsininhibitor column.Anti-interferon antibody column was established by couplingthe adsorbed anti-interferon antibody to Sepharose 4B.Crude Namalva interferonwas purified by antibody affinity chromatography in one step to a specific activityof 8×10~6 to 8×10~7 units/mg protein with the recovery of over 100%.Peak ofpurified interferon activity in elution was seen mostly in fractions 1-3(3-8ml).Similar results were obtained in purification of human leukocyte interferon(HuLeIFN)by this column.The polyclonal antibody column could purifyrecombined leukocyte interferon-xD(LrIFN-αD)to homogeneity as analyzed onSDS-polyacrylamide gel electrophoresis.
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