哺乳期母源应激对子代小鼠青春期神经行为的影响

目的探讨哺乳期母源应激对子代小鼠青春期神经行为发育的影响。方法哺乳期母鼠随机分为应激组、分离组、对照组，小鼠出生7d后，应激组母鼠每天进行5min的冷水游泳应激，持续14d，采用强迫游泳、悬尾实验、自发活动等方法对子代小鼠青春期（35d）体质量、神经行为进行评定。结果哺乳期母源慢性应激显著增加青春期子代小鼠对应激的易感性，表现为对强迫游泳漂浮不动时间的显著增加雌性：应激组、分离组和对照组分别为（139±6）s，（138±9）S和（96±9）S；雄性：3组分别为（139±9）s，（112±9）S和（96±9）s]和悬尾测试中小鼠不动时间的增加雌性：应激组、分离组和对照组分别为（127±8）s，（123±8）S和（98±6）S；雄性：3组分别为（141±7）s，（105±5）s和（92±6）s]；同时发现应激易感性的改变存在性别差异，雌性个体对哺乳期母源慢性应激更加敏感。结论哺乳期母源应激可能是子代青春期抑郁发生的重要影响因素，为青春期抑郁症的早期预防和干预拓宽了思路。

The effects of lactational maternal stress on neuronal behavior of adolescent offspring in mice

Objective To explore the effects of lactational maternal stress on neurobehavioral develop- ment of offsprings. Methods Maternal mice were divided into normal control, maternal deprivation and maternal stress group. A 5-min/day cold water swimming was used as maternal stress for continuously of 14 day since the 7th postnatal day. The increasing bodyweight and neurobehavior of adolescent offspring mice were assessed by forced swimming test, tail suspension test and locomotor activity in the 35th postnatal day. Results Maternal chro- nic stress in laetational stage significantly increased the vulnerability of offspring to acute stress, which was reflec- ted by significantly increased immobility time of adolescent offspring in forced swimming test （female：（139±6） s, （ 138± 9） s, （ 96± 9） s respectively ; male ： （139±9） s, （112±9） s, （96±9） s respectively） and in tail suspension test （ femal ： （127_＋8） s, （123±8） s, （98±6） s respectively; male ： （ 141 ±7） s, （105±5） s, （92±6） s respectively）. Mean- while, sexual difference occurred for adolescent offspring to acute stress, and the female offspring were more vulner- able to acute stress. Conclusion Lactational maternal stress may be important factors for development of adoles- cent depression.Our findings highlight the area of early prevention and intervention for adolescent depression .