宫腔粘连模型的建立与评价及对生育力的影响

目的：苯酚胶浆法建立宫腔粘连动物模型并对其生育力进行评价。方法：将大鼠60只，随机分为造模组（30只）、假手术组（15只）、正常对照组（15只），苯酚胶浆法建立宫腔粘连模型。造模术后10d，取造模组15只、假手术组7只、正常对照组7只处死，肉眼观察其官腔改变，并进行大体分级；各组宫腔标本进行HE和Masson染色对其进行纤维化半定量评分，并对比各组的变化；剩余大鼠自然妊娠，妊娠第10天开腹计数子宫内胚胎数目作为评价生育力的指标。结果：造模侧宫腔狭窄甚至闭塞，宫腔粘连纤维化半定量评分显示：造模组（6．6±0．98分）和正常组（0分）、假手术组（-0．5±1．91分）差异有统计学意义（P〈0．001），假手术组和正常组差异无统计学意义（P〉0．05）；妊娠胚胎数造模组（0．2±0．4个）和假手术组（6．3±2．9个）、正常组（6．3±1．8个）差异有统计学意义（P〈0．001）；假手术组和正常组差异无统计学意义（P〉0．05）。结论：苯酚胶浆法可成功建立官腔粘连的动物模型；宫腔粘连的大鼠生育力明显降低。

Establishment and evaluation of a rat model of intrauterine adhesion

Objective ： To establish a rat model of intrauterine adhesion with phenol mucilage and evaluate the fertility of model rats. Methods ： Sixty SD female rat without pregnancy were randomly divided into three group ： operation group （ OG, n = 30） , sham operated groups （ SOG, n = 15 ） and normal group （ n = 15 ）. A rat model of intrauterine adhesions was established with phenol mucilage. Two weeks later, animals in OG （n = 15） , SOG （n =7） and the normal group （n =7） were sacrificed. Changes of uterine in the gross anatomy and histology with hematoxylin eosin and Masson stains were evaluated. Animals of OG （n = 15 ）, SOG （n = g ） and the normal group （n = g ） were bred and sacrificed during 10 days of pregnancy, and numbers of implanted fetus were counted to evaluate the fertility. Results： Synechia was observed at fight uterus （the operating side） of OG. The score of endometrial fibrosis in OG （6.6 ±0.98） was higher than that in any other groups （0, - 0.5 ±1.91, P 〈 0. 001 ）, and those in the latter were similar （ P 〉 0.05 ）. Numbers of implanted fetus in OG （ 0.2 ±0.4 ） were more than those in any other groups （6.3 ±2.9, 6.3 ±1.8, P 〈 0. 001 ） , and those in the latter were similar（P 〉 0.05 ）. Conclusion ： The rat model of intrauterine adhesion with phenol mucilage is established successfully and the fertility of model rats is re- duced.