温脾汤提取物对小鼠记忆功能及脑内SOD和MDA的影响

目的探讨温脾汤提取物对正常和脑缺血再灌小鼠记忆功能及脑内超氧化物歧化酶（SOD）及丙二醛（MDA）的影响。方法将小鼠分为正常组、假手术组、模型组、温脾汤水提物和醇提物各三个剂量组（1．0g/kg、0．5g/kg、0．25g/kg）。采用反复结扎双侧颈总动脉制备小鼠脑缺血再灌损伤模型。利用跳台和避暗实验，测试温脾汤提取物连续灌胃10d，对正常和脑缺血再灌小鼠记忆行为的影响，并检测小鼠脑内SOD活性和MDA含量。结果跳台实验中，与生理盐水组相比，温脾汤水提物和醇提物各剂量导致小鼠的潜伏期延长、错误次数减少，以高剂量最明显（P〈0．05）潜伏期：（187．17±99．00）S，（270．73±44．73）S，（260．45±63．78）S；错误次数（1．75±1．71）次，（0．45±0．69）次，（0．75±0．97）次]；避暗实验中，与模型组相比，水提物高、中剂量和醇提物高剂量能使脑缺血再灌小鼠的潜伏期延长及错误次数减少（P〈0．05）潜伏期（157．15±84．91）S，（250．63±58．98）S，（251．12±78．22）s，（245±84．82）s；错误次数（2．15±1．22）次，（0．75±0．89）次，（0．75±1．16）次，（0．625±0．92）次]；温脾汤水提物高、中剂量小鼠脑内SOD活性较模型组明显增强（（P〈0．05），MDA的含量与模型组相比明显降低（P〈0．05，P〈0．01）。结论温脾汤提取物可提高正常小鼠的记忆功能，改善脑缺血再灌小鼠记忆功能，其机制可能与恢复脑组织SOD活力及减少MDA含量有关。

Influence of the extract of wen-pi-tang on memory function of mice and SOD and MDA in brain

Objective To explore the influence of the extract of wen-pi-tang on the memory and SOD and MDA of normal mice and cerebral isehemia reperfusion mice.Method The mice were divided into normal group, sham operation group, model group, aqueous extract and alcohol extract of wen-pi-tang （1.0 g/kg, 0.5 g/kg, 0.25 g/kg） .Model mice with cerebral ischemia reperfusion were made by blocking bilateral common carotid artery. The influence on memory behavior of normal mice and mice with cerebral ischemia reperfusion were tested by gavage for 10 days with extract of wen-pi-tang through step-down and step-through tests.Then SOD activity and content of the MDA were tested. Result In step-down test, compared with normal group, extract of wen-pi-tang with all doses made normal mice＇ latency longer, error frequency fewer, with high does clearest （P〈 0.05 ） （ latency ： （ 187.17 ± 99. 00） s, （ 270.73±44.73 ） s, （ 260.45±63.78 ） s, error frequency（ 1.75± 1.71 ） times, （0.45±0.69） times , （ 0.75±0.97 ） times） ;in step-through test, compared with normal group, aqueous extract with high and middle doses and alcohol extract with longe doses could made latency of cerebral ischemia reperfusion mice longer and error frequency fewer （ latency（ 157.15±84.91 ） s, （250.63±58.98） s, （251.12±78.22） s, （245±84.82） s, error frequency（ 2.15± 1.22） times, （0.75±0.89）times, （0.75±1.16） times, （0.625±0.92） times） （P〈0.05） ;extract of wen-pi-tang with high and middle does increased SOD activity obviously compared with normal group（P〈0.05）, and the content of MDA was obviously lower compared with normal group（P〈0.05, P〈0.01）. Conclusion Extracts of wen-pi-tang can im- prove the memory function of normal mice, and the memory of cerebral ischemia reperfusion mice.The mechanism can be related to the increase of SOD activity and the decrease of the content of MDA.