基于血浆代谢组学分析金欣口服液治疗小鼠RSV肺炎的作用机制

目的:通过GC-MS技术分析BALB/c小鼠血浆内源性代谢产物的相对含量变化,阐明金欣口服液治疗呼吸道合胞病毒(RSV)肺炎的作用机制。方法:RSV滴鼻感染BALB/c小鼠建立RSV肺炎模型,首次滴鼻2 h后予金欣口服液(剂量27.6 g·kg-1·d-1)灌胃治疗,连续滴鼻3 d后各组处死5只小鼠取肺组织行组织病理学检查,连续给药7 d后采集各组小鼠的血浆,运用GC-MS检测(m/z 50~500)血浆内源性代谢物及其相对含量的变化,分析其可能参与的代谢通路。结果:RSV肺炎小鼠的血浆中共测得32种内源性代谢产物,其中乳酸、尿素、谷氨酰胺、葡萄糖、花生四烯酸和1,5-脱水山梨醇为差异代谢产物,主要与花生四烯酸、谷氨酰胺、丙酮酸的代谢通路有关。结论:金欣口服液治疗RSV肺炎可能与调节RSV感染导致的代谢紊乱相关。

Mechanism Analysis of Jinxin Oral Liquid Against Respiratory Syncytial Virus Pneumonia Based on Plasma Metabonomics

Objective: To detect the relative content changes of endogenous metabolites in plasma of BALB/c mice by GC-MS technology, and to explore the mechanism of Jinxin oral liquid against respiratory syncytial virus (RSV) pneumonia in vivo. Method: BALB/c mice were challenged intranasally with RSV to establish the RSV pneumonia model, then intragastric administration of Jinxin oral liquid with dose of 27.6 g · kg^-1 · d^-1.After 3 days of inoculation, five mice in each group were hanged and inferior lobe of right lung were collected to take histopathological examination.After continuously given Jinxin oral liquid for 7 days, plasma were collected to detect the endogenous metabolites by GC-MS (m/z 50-500) and possible metabolic pathways concerned were analyzed. Result: Thirty-two endogenous metabolites in plasma were detected, arachidonic acid, glutamine, lactic acid, D-glucose, urea and 1, 5-anhydro-D-sorbitol were differential metabolites among them.Metabolic pathways of archidonic acid, glutamine and pyruvate were the most concerned. Conclusion: The antiviral effect of Jinxin oral liquid against RSV pneumonia may be related to regulation of metabolic disorder caused by RSV.