苦豆子不同部位总生物碱提取物指纹图谱分析

目的:研究苦豆子不同部位总生物碱提取物的HPLC指纹图谱,为科学评价及有效控制苦豆子总生物碱提取物的质量提供依据。方法:采用HPLC-DAD方法,对不同主产区的16批苦豆子种子(苦豆籽)和地上部分(苦豆草)总生物碱提取物进行指纹图谱研究,采用相似度评价对指纹图谱进行分析,同时运用聚类分析及主成分分析对其进行模式识别。结果:分别建立苦豆籽和苦豆草提取物对照指纹图谱,确定共有峰,并指认色谱峰。通过聚类分析和主成分分析将16批药材总生物碱提取物按不同产地分为3类。结论:苦豆籽和苦豆草提取物的指纹图谱特征性及专属性良好,可用于全面控制苦豆籽和苦豆草总生物碱的质量。

HPLC Fingerprint of Total Alkaloids from Different Parts of Sophora alopecuroides

Objective: To study the HPLC fingerprint of the total alkaloids from different parts of the Sophora alopecuroides, and provide the basis for scientific evaluation and effective control of the quality of total alkaloids in S. alopecuroides. Method: Totally 16 batches of S. alopecuroides from different producing areas and the total alkaloids were used to gain fingerprints by HPLC-DAD. The obtained data was analyzed by similarity evaluation, and the pattern recognition was carried out by cluster analysis and principal component analysis. Result: Fingerprints of S. alopecuroides fructus and herba S. alopecuroides extracts were established; common peaks were determined, and the chromatographic peaks were identified. Through cluster analysis and principal component analysis, the total alkaloids extract of the 16 batches Chinese medicinal materials were divided into 3 groups according to different producing areas. Conclusion: S. alopecuroides fructus and herba S. alopecuroides extracts had good fingerprint features and specificity, which could be used for the overall quality control of the total alkaloids from S. alopecuroides fructus and herba S. alopecuroides.