金银花活性成分与生态因子相关性研究

目的:分析比较产地生态因子对金银花活性成分的影响,为金银花适宜种植区域确定提供依据。方法:于2014年5—7月份在全国金银花种植区海拔在4~2 100 m采得11个海拔高度上的金银花样本,经HPLC分析得到10种活性成分含量,同时获取各样地相应的气候因子,用SPSS 17.0统计软件对所得数据进行相关性分析和逐步线性回归分析。结果:不同产地金银花活性成分含量差异显著;海拔高度是影响绿原酸与木犀草苷的主导生态因子,随着金银花种植区海拔的升高,绿原酸和木犀草苷含量呈增加趋势;咖啡酸含量与7月份最高温呈显著正相关,与日照时数呈显著正相关;异绿原酸A含量与降雨量呈显著正相关;槲皮素含量与1月份最低温度呈显著正相关。结论:根据本实验研究结果推测,金银花种植区随着海拔升高,花期温度降低,有利于绿原酸和木犀草苷合成。

Correlation Between Bioactive Components of Lonicerae Japonicae Flos and Ecological Factors

Objective: To provide the fundamental basis for determining suitable planting areas of Lonicerae Japonicae Flos by analyzing and comparing the effect of ecological factors on active ingredients of Lonicerae Japonicae Flos. Method: Totally 11 samples of Lonicerae Japonicae Flos were collected from different areas with altitudes ranging from 4-2100 m, 10 bioactive contents of Lonicerae Japonicae Flos were obtained by HPLC. Meanwhile, climatic factors of different areas were collected, and the resulting data were statistically analyzed using SPSS 17.0 statistical software for the correlation analysis and the stepwise linear regression analysis. Result: There were significant differences among active ingredients of Lonicerae Japonicae Flos from different areas. Altitude is the dominant ecological factor influencing on the contents of chlorogenic acid and luteoloside. With the increase of altitude, the contents of chlorogenic acid and luteoloside showed a trend of increase. The content of caffeic acid was significantly positively related with the highest temperature in July, and negative related with the sunshine time. The content of isochlorogenic acid A and rainfall were significantly negative related, the content of quercetin and the minimum temperature in January showed a significant positive correlation. Conclusion: According to the results of this experimental study, we can speculate that with the increase of altitude in the planting areas of Lonicerae Japonicae Flos, the flowering temperature reduces, which is more conducive to the synthesis of chlorogenic acid and luteoloside.