神农香菊挥发油GC-MS指纹图谱

目的:建立了神农香菊挥发油GC-MS指纹图谱分析测定方法,为神农香菊药材的质量控制提供参考。方法:采用水蒸气蒸馏法提取神农香菊挥发油,用GC-MS联用技术对神农香菊进行指纹图谱测定,并采用国家药品监督管理局推荐的中药指纹图谱计算软件进行计算,建立神农香菊挥发油的共有指纹图谱。结果:神农香菊挥发性成分中含有23个特征性指标成分,初步建立了以此23个共有峰为特征指纹信息的GC-MS指纹图谱。结论:该方法具有较好的重复性、精密度和稳定性(RSD均5%),建立的神农香菊挥发油指纹图谱为其质量控制奠定了基础。

GC-MS Fingerprint of Volatile Oil from Dendranthema indicum var. aromaticum

Objective: To establish GC-MS fingerprint determination method of volatile oil from Dendranthema indicum var. aromaticum, and provide the reference for the quality control of D. indicum var. aromaticum medicinal materials. Method: The steam distillation method was used to extract the volatile oil from D. indicum var. aromaticum; the combined technology of GC-MS was used to determin its fingerprints, and Fingerprint Calculation Software of traditional Chinese medicine recommended by State Drug Administration was used for calculation, in order to establish the common fingerprints of volatile oil from D. indicum var. aromaticum. Result: There were 23 main characteristic components in the volatile oil from D. indicum var. aromaticum, and GC-MS fingerprint with these 23 common peaks was established preliminarily. Conclusion: The method is repeatable, accurate and stable, which can be used for quality control of the D. indicum var. aromaticum.