栀子生品及炮制品对CCl4致大鼠急性肝损伤保护作用的比较

目的:比较栀子生品及炮制品对四氯化碳(CCl_4)致急性肝损伤大鼠的保护作用差异。方法:大鼠按体重随机等分为9组,分别为正常组,阳性组(联苯双酯35 mg·kg-1),模型组,生栀子高、低剂量组(2.0,1.0 g·kg-1),炒栀子高、低剂量组(2.0,1.0 g·kg-1),焦栀子高、低剂量组(2.0,1.0 g·kg-1),造模12 h后眼球取血,测定血清中天门冬氨酸转移酶(AST),丙氨酸氨基转移酶(ALT),乳酸脱氢酶(LDH),总蛋白(TP)的活性,测定肝组织中超氧化物歧化酶(SOD)的活性和丙二醛(MDA)的含量。观察肝脏病理组织学变化。结果:与正常组比较,模型组显著升高AST,ALT,LDH水平和MDA含量,降低了TP的水平及SOD活性。肝细胞排列紊乱,细胞肿胀坏死明显。与模型组比较,栀子生品及炮制品组可显著降低大鼠血清中ALT,AST,LDH活性,其中生栀子组与炒栀子组效果较佳;各给药组均可增高SOD活性,降低MDA水平。各栀子给药组与模型组比较血清TP水平无显著性差异。各给药组肝细胞坏死、变性及炎性细胞浸润程度均有所改善,且生品组与炒品组改善较为显著。结论:栀子生品、炒品、焦品对CCl_4急性肝损伤大鼠均有较好的保护作用,而生品、炒品具有较优的保肝降酶、抗氧化作用,且炒品能够改善生品苦寒之性的缺点,缓和药性,临床应用可能更宜使用炒栀子。

Comparison Between Protective Effect of Raw and Processed Products of Gardeniae Fructus on Acute Hepatic Injury Induced by CCl4 in Rats

Objective: To compare protective effect of raw and processed products of Gardeniae Fructus on acute hepatic injury induced by CCl₄ in rats. Method: SD rats were evenly randomized into 9 groups by weight, such as normal group, bifendate group (35 mg · kg^-1), model group, low and high dose of raw products of Gardeniae Fructus (2.0, 1.0 g · kg^-1) groups, low and high dose of fried products of Gardeniae Fructus (2.0, 1.0 g · kg^-1) groups, low and high dose of stir-baked products of Gardeniae Fructus (2.0, 1.0 g · kg^-1) groups.Twelve hours after modeling, serum levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH) and total protein (TP) were examined.Super oxide dismutase (SOD) activity and malondialdehyde (MDA) content in liver tissue were determined.Histopathological changes of liver were observed. Result: Compared with the normal group, the model group significantly increased levels of AST, ALT, LDH, MDA and decreased level of TP and SOD activity.Liver cells arranged disordered, swelling and necrosis of liver cells were obvious.Compared with the model group, levels of AST, ALT and LDH in serum of raw and processed products groups decreased significantly, the raw and fried products groups were better.Raw and processed products groups increased activity of SOD and decreased the level of MDA significantly.There were no significant difference between the model group and administration groups in serum TP level of rats.Each administration groups reduced the number of degeneration, necrosis of liver cells and improve inflammatory cells infiltration. Conclusion: The raw, fried and stir-baked products of Gardeniae Fructus have an effective protection on acute hepatic injury induced by CCl₄, the the raw and fried products are better in reducing enzyme level and antioxidant capacity.The fried products can decrease the bitter and cold of raw products.So the fried products of Gardeniae Fructus may be more appropriate in clinical application.