十二味穿甲片对大鼠免疫性肝纤维化模型的影响

目的:研究十二味穿甲片对大鼠免疫性肝纤维化模型的影响,并探讨其相关的作用机制。方法:将雄性SD大鼠随机分为正常组,模型组,阳性药组(秋水仙碱,0.1 mg·kg-1),中药十二味穿甲片2.8,1.4,0.7 g·kg-1剂量组。除正常组外,其余各组采用猪血清制备大鼠免疫性肝纤维化模型,造模同时给药进行抗肝纤维化干预,共6周。通过计算体重、肝脏指数,检测肝功能丙氨酸转氨酶(ALT),天冬氨酸转氨酶(AST),总蛋白(TP),白蛋白(ALB),球蛋白(GLO)水平和白球比(A/G),酶联免疫吸附测定(ELISA)法检测血清透明质酸(HA)含量。苏木素-伊红(HE)染色以观察肝组织病理变化及马松(Masson)三色染色观察胶原纤维增生情况,评价药物的抗纤维化作用。结果:与正常组比较,模型组大鼠血清ALT,HA水平明显升高,肝指数明显升高(P0.01),ALB,A/G明显降低(P0.01),模型组肝组织的病变较明显;与模型组比较,十二味穿甲片除对血清AST,GLO的变化无明显影响外,能够显著降低肝纤维化大鼠血清ALT和纤维化指标HA水平(P0.01),不同程度地升高血清TP,ALB和A/G,并能显著地减轻肝组织的病变,尤以十二味穿甲片2.8 g·kg-1剂量效果最显著。结论:十二味穿甲片可明显改善模型大鼠肝纤维化程度,显示出一定的抗肝纤维化作用。

Effect of Shierwei Chuanjia Tablets on Model of Immunological Hepatic Fibrosis in Rats

Objective: To study the effect of Shierwei Chuanjia tablets on immunological hepatic fibrosis in rat models. Method: The male SD rats were randomly divided into normal group, model group, positive drug group (colchicine tablets, 0.1 mg · kg^-1), and Shierwei Chuanjia tablets groups (2.8, 1.4, 0.7 g · kg^-1). In all the other groups except normal group, pig serum was used to prepare the rat models of immunological hepatic fibrosis. At the same time, the rats received corresponding anti hepatic fibrosis interventions for a total of 6 weeks. The effects of drugs were observed by calculating the body mass and liver index, detecting liver function alanine aminotransferase (ALT), aspartate aminotransferase (AST), total protein(TP), albumin(ALB), globulin(GLO), and albumin globulin ratio (A/G). hyaluronate (HA) levels in serum were measured by using ELISA. HE staining was used to observe the pathological changes of liver tissues and Masson staining was used to observe the proliferation of collagen fibers. Result: As compared with the normal group, ALT and HA levels in serum of model rats were significantly increased, and liver indexes were also significantly increased (P<0.01); ALB and A/G were significantly decreased (P<0.01); the pathological changes of liver tissues were more obvious in model group. As compared with the model group, The other groups of Shierwei Chuanjia tablets had no significant effect on the changes of serum AST and GLO levels, but could significantly decrease the serum ALT and HA levels in liver fibrosis rats (P<0.01), increase the levels of serum TP, ALB and A/G, and can significantly reduce the pathological changes of liver tissues, with the most significant effect in 2.8 g · kg^-1Shierwei Chuanjia tablets group. Conclusion: Shierwei Chuanjia tablets can significantly improve the hepatic fibrosis in model rats, showing certain anti-hepatic fibrosis effect.