Protective effects of Terminalia arjuna against Doxorubicin-induced cardiotoxicity |
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Authors: | Singh Gurvinder Singh Anu T Abraham Aji Bhat Beena Mukherjee Ashok Verma Ritu Agarwal Shiv K Jha Shivesh Mukherjee Rama Burman Anand C |
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Affiliation: | Dabur Research Foundation, 22 Site IV, Sahibabad, Ghaziabad 201010, U.P., India. |
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Abstract: | Terminalia arjuna has been marked as a potential cardioprotective agent since vedic period. The present study was aimed to investigate the effects of butanolic fraction of Terminalia arjuna bark (TA-05) on Doxorubicin (Dox)-induced cardiotoxicity. Male wistar rats were used as in vivo model for the study. TA-05 was administered orally to Wistar rats at different doses (0.42 mg/kg, 0.85 mg/kg, 1.7 mg/kg, 3.4 mg/kg and 6.8 mg/kg) for 6 days/week for 4 weeks. Thereafter, all the animals except saline and TA-05-treated controls were administered 20 mg/kg Dox intraperitonially. There was a significant decrease in myocardial superoxide dismutase (38.94%) and reduced glutathione (23.84%) in animals treated with Dox. Concurrently marked increase in serum creatine kinase-MB (CKMB) activity (48.11%) as well as increase in extent of lipid peroxidation (2.55-fold) was reported. Co-treatment of TA-05 and Dox resulted in an increase in the cardiac antioxidant enzymes, decrease in serum CKMB levels and reduction in lipid peroxidation as compared to Dox-treated animals. Electron microscopic studies in Dox-treated animals revealed mitochondrial swelling, Z-band disarray, focal dilatation of smooth endoplasmic reticulum (SER) and lipid inclusions, whereas the concurrent administration of TA-05 led to a lesser degree of Dox-induced histological alterations. These findings suggest that butanolic fraction of Terminalia arjuna bark has protective effects against Dox-induced cardiotoxicity and may have potential as a cardioprotective agent. |
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Keywords: | CKMB, creatine kinase Dox, doxorubicin DTNB, [5,5-dithiobis(2-nitrobenzoic acid)] DPPH, [1,1-diphenyl-2-picrylhydrazyl] GSH, reduced glutathione MDA, malonyldialdehyde mg, milligram NADH, nicotinamide adenine dinucleotide-reduced disodium salt ROS, reactive oxygen species SER, smooth endoplasmic reticulum SDS, sodium dodecyl sulphate SOD, superoxide dismutase TBA, thiobarbituric acid TBARS, thiobarbituric acid reactive substances TCA, trichloroacetic acid IR, ischemic reperfusion |
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