脉冲电磁场联合电针疗法对去卵巢大鼠骨质疏松的影响

目的探索脉冲电磁场联合电针疗法对去卵巢大鼠骨质疏松的影响。方法将50只SD雌性大鼠随机分为假手术组、去势组、脉冲电磁场组、电针组和联合组,每组10只。除假手术组外,各组大鼠行双侧卵巢切除术,假手术组大鼠只切除卵巢周围与卵巢大小相仿的脂肪组织。去除卵巢术后1周,假手术组和去势组不作任何干预,脉冲电磁场组给予脉冲电磁场治疗、电针组给予电针治疗,联合组同时给予脉冲电磁场和电针治疗。干预12周后处死实验动物,取左股骨和L5椎体进行骨密度检测及生物力学检测；取左胫骨观察组织形态学变化。结果脉冲电磁场、电针和联合治疗能增加去势大鼠左股骨骨密度（P〈0.01,P〈0.05）；而脉冲电磁场组、电针组和联合组3组之间股骨骨密度无统计学差异（P＆gt；0.05）；脉冲电磁场、电针和联合治疗能增加L5椎体骨密度（P〈0.01）；与脉冲电磁场组和电针组比较,联合组L5椎体骨密度明显增高（P〈0.05）。脉冲电磁场、电针和联合疗法均能增加骨小梁面积、骨小梁数量和骨小梁链接数,减少骨髓腔的大小。脉冲电磁场和联合治疗能增加去势大鼠股骨最大载荷（P〈0.05）；而脉冲电磁场组、电针组和联合组3组之间最大载荷无统计学差异（P＆gt；0.05）。脉冲电磁场、电针和联合治疗能增加L5椎体最大载荷（P〈0.01）；与脉冲电磁场组和电针组比较,联合组最大载荷明显增高（P〈0.05,P〈0.01）。脉冲电磁场、电针和联合治疗能增加L5椎体破坏能（P〈0.01）；与脉冲电磁场组和电针组比较,联合组破坏能明显增高（P〈0.01）。结论脉冲电磁场联合电针疗法在增加腰椎椎体骨密度,改善腰椎椎体骨生物力学性能方面优于脉冲电磁场或电针疗法单独使用。

Effects of Combination Treatment with Pulsed Electromagnetic Fields and Electroacupuncture on Ovariectomy-induced Osteoporosis in Rats

Objective To explore whether combination treatment with pulsed electromagnetic fields（ PEMFs） and electroacupuncture（ EA） have additive benefits on bone mineral density（ BMD） and biomechanical properties in OVX rats.Methods Fifty 3-month old female Sprague-Dawley rats were assigned to one of five groups： sham-operated control（ Sham）,ovariectomy（ OVX）,ovariectomy with PEMFs treatment（ PEMFs）,ovariectomy with EA treatment（ EA）,and ovariectomy with PEMFs ＋ EA treatment（ PEMFs ＋ EA）. After 12-week treatments,bone mineral density,histomorphometric parameters,and bone biomechanical properties were examined. Results PEMFs,EA and combination treatment significantly increased BMD of the femur（ P 〈 0. 01,P 〈 0. 05） compared to OVX group. There was no significant difference in the femur BMD among the five groups（ P 〈 0. 05）. PEMFs,EA and combination treatment significantly increased BMD of L5vertebral body（ P 0. 01） compared to OVX group. What＇s more,combination treatment with PEMFs and EA was more effective than PEMFs or EA alone for improving BMD in L5 vertebral body（ P 0. 05）. The biomechanical three-point bending experiment showed that PEMFs and combination treatment significantly increased maximum load in the left femur compared to OVX group（ P 〈 0. 05）. There was no significant difference in maximum load of the left femur among the five groups（ P〈 0. 05）. The compression test showed that PEMFs,EA and combination treatment significantly increased maximum load（ P 〈 0. 01） and energy to failure（ P 〈0. 01） in L5 vertebral body compared to OVX group. What＇s more,combination treatment was more effective than PEMFs or EA alone for improving maximum load（ P 〈 0. 05,P〈 0. 01） and energy to failure（ P 〈 0. 01） in L5 vertebral body. Conclusions Combination treatment with PEMFs and EA have additive benefits on BMD and bone biomechanical properties in the lumbar vertebral body.