转录因子Yin Yang 1(YY1)在食管癌中的表达及其对食管癌细胞功能的影响

目的:探讨转录因子Yin Yang 1(YY1)在正常食管组织和食管鳞癌组织中的表达及其在食管癌发生发展中的作用?方法:免疫组化染色检测15例正常食管组织,39例食管癌旁组织和88例食管癌组织样品中YY1蛋白表达水平;噻唑蓝(MTT)和克隆实验检测食管鳞癌细胞(TE-1细胞)增殖和生长变化;蛋白质印迹(Western blot)技术检测转染后转录因子YY1和p21表达水平;细胞划痕实验和Transwell实验检测TE-1细胞迁移?侵袭能力的变化?结果:与正常食管组织和食管癌旁组织相比,YY1 蛋白在食管鳞癌组织样品中的表达显著提高?此外,转染了YY1过表达载体后明显抑制TE-1细胞生长,而干扰YY1表达则可促进细胞的生长;同时伴随着YY1靶基因p21(也称p21WAF1/Cip1)的变化?TE-1细胞转染YY1过表达载体后,细胞的侵袭?迁移能力增强?结论:食管鳞癌组织中YY1表达显著上调,YY1可调节细胞的生长和转移侵袭能力?因此,YY1有望成为食管癌诊断和发展的分子标志物,也有可能是食管癌基因治疗的一个潜在靶点?

The expression of transcription factor Yin Yang 1 (YY1) and its function in esophageal squamous cell carcinoma

Yin Yang -1(YY1) is a member of the zinc finger factor GLI-Krüppel family. Objective:To investigate the gene expression and the function of YY1 in normal esophageal tissues and esophageal squamous carcinoma. Methods:The protein expression of YY1 in 88 esophageal squamous cell carcinomas,39 tumor adjacent tissues and 15 normal esophageal specimens was determined by immunohistochemistry (IHC). Proliferation and growth changes of esophageal squamous carcinoma cells were measured by MTT and colony formation. YY1 and p21 protein expressions were determined by Western blotting assays. TE-1 cell invasion and migration were determined by cell scratch experiment and Transwell invasive assays. Results:Compared to normal esophageal and tumor adjacent tissues,the expression of YY1 was significantly higher in esophageal squamous cell carcinomas. Furthermore,TE-1 cells overexpressing of YY1 significantly suppressed cell growth and downregulation of YY1 promoted cell growth. Meanwhile,a change of YY1 target gene p21 (p21WAF1/Cip1) was found. TE-1 cells overexpressing YY1 promoted cell invasion and migration. Conclusion:The expression of YY1 was significantly increased and regulated the growth,invasion and migration ability of esophageal squamous cells. Therefore,YY1 is expected to be a molecular marker in the diagnosis and development of esophageal squamous cell carcinoma,and also a potential target for gene therapy of esophagus cancer.