| 树鼩骨髓间充质干细胞体外分离培养及成脂成骨诱导 |
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| 引用本文： | 陆彩霞,李晓飞,王文广,孙晓梅,仝品芬,匡德宣,代解杰. 树鼩骨髓间充质干细胞体外分离培养及成脂成骨诱导[J]. 中国比较医学杂志, 2014, 24(3): 10-13 |
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| 作者姓名： | 陆彩霞 李晓飞 王文广 孙晓梅 仝品芬 匡德宣 代解杰 |
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| 作者单位： | 中国医学科学院, 北京协和医院医学生物学研究所树鼩种质资源中心, 昆明 650118;云南省重大传染病疫苗研发重点实验室, 昆明 650118;中国医学科学院, 北京协和医院医学生物学研究所树鼩种质资源中心, 昆明 650118;云南省重大传染病疫苗研发重点实验室, 昆明 650118;中国医学科学院, 北京协和医院医学生物学研究所树鼩种质资源中心, 昆明 650118;云南省重大传染病疫苗研发重点实验室, 昆明 650118;中国医学科学院, 北京协和医院医学生物学研究所树鼩种质资源中心, 昆明 650118;云南省重大传染病疫苗研发重点实验室, 昆明 650118;中国医学科学院, 北京协和医院医学生物学研究所树鼩种质资源中心, 昆明 650118;云南省重大传染病疫苗研发重点实验室, 昆明 650118;中国医学科学院, 北京协和医院医学生物学研究所树鼩种质资源中心, 昆明 650118;云南省重大传染病疫苗研发重点实验室, 昆明 650118;中国医学科学院, 北京协和医院医学生物学研究所树鼩种质资源中心, 昆明 650118;云南省重大传染病疫苗研发重点实验室, 昆明 650118 |
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| 基金项目： | 国家科技支撑计划（2011BAI15B01-21；2012BAI39B01；2014BAI01B01）。 |
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| 摘 要： | 目的探讨树鼩骨髓间充质干细胞(BM-MSCs)的体外分离、传代及定向诱导为脂肪细和成骨细胞的可行性。方法通过密度梯度离心联合贴壁培养法对树鼩骨髓间充质干细胞进行体外分离、扩增、纯化,倒置相差显微镜进行形态学观察。用成脂诱导液(DMEM/F12+10%FBS+100 U/mL青霉素+100μg/mL链霉素+1.0μmol/L地塞米松+0.2 mmol/L吲哚美辛+0.01 mg/mL胰岛素+0.5 mmol/L IBMX)和成骨诱导液(高糖DMEM+10%FBS+100 U/mL青霉素+100μg/mL链霉素+50 ng/mL BMP-2)对分离的树鼩BM-MSCs分别定向诱导为脂肪细胞和成骨细胞。结果原代和传代细胞为梭形或三角形,可增殖形成克隆。BM-MSCs成脂诱导后油红O染色细胞内出现红色脂滴,成骨诱导后茜素红染色可观察到矿化结节。结论密度梯度离心联合贴壁培养法分离培养树鼩BM-MSCs简便可行,获得的BM-MSCs可体外诱导分化为脂肪细胞和成骨细胞。
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| 关 键 词： | 树鼩 骨髓间充质干细胞 细胞培养 成脂诱导 成骨诱导 |
| 修稿时间： | 2014-02-12 |
Isolation,culture, adipocgenic and osteogenic induction of Tupaia bone marrow mesenchymal stem cells |
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| LU Cai-xi,LI Xiao-fei,WANG Wen-guang,SUN Xiao-mei,TONG Pin-fen,KUANG De-xuan and DAI Jie-jie. Isolation,culture, adipocgenic and osteogenic induction of Tupaia bone marrow mesenchymal stem cells[J]. Chinese Journal of Comparative Medicine, 2014, 24(3): 10-13 |
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| Authors: | LU Cai-xi LI Xiao-fei WANG Wen-guang SUN Xiao-mei TONG Pin-fen KUANG De-xuan DAI Jie-jie |
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| Affiliation: | The Center of Tree shrew Germplasm Resources, Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College, Kunming 650118, China;Yunnan Key Laboratory of Vaccine Research & Development on Severe Infection Diseases, Kunming 650118, China;The Center of Tree shrew Germplasm Resources, Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College, Kunming 650118, China;Yunnan Key Laboratory of Vaccine Research & Development on Severe Infection Diseases, Kunming 650118, China;The Center of Tree shrew Germplasm Resources, Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College, Kunming 650118, China;Yunnan Key Laboratory of Vaccine Research & Development on Severe Infection Diseases, Kunming 650118, China;The Center of Tree shrew Germplasm Resources, Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College, Kunming 650118, China;Yunnan Key Laboratory of Vaccine Research & Development on Severe Infection Diseases, Kunming 650118, China;The Center of Tree shrew Germplasm Resources, Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College, Kunming 650118, China;Yunnan Key Laboratory of Vaccine Research & Development on Severe Infection Diseases, Kunming 650118, China;The Center of Tree shrew Germplasm Resources, Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College, Kunming 650118, China;Yunnan Key Laboratory of Vaccine Research & Development on Severe Infection Diseases, Kunming 650118, China;The Center of Tree shrew Germplasm Resources, Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College, Kunming 650118, China;Yunnan Key Laboratory of Vaccine Research & Development on Severe Infection Diseases, Kunming 650118, China |
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| Abstract: | Objective To study the isolation,culture, adipogenic and osteogenic induction Tupaia bone marrow mesenchymal stem cells(BM-MSCs). Method The BM-MSCs from tupaia were isolated and expended by combination of gradient centrifugation and adherence culture, then subcultured and observed for morphology under inverted phase contrast microscope. BM-MSCs were induced to adipocytes.and osteoblasts in vitro Result Cells were spindle or triangle-shaped,and clone proliferation.Cells were successfully induced into adipocytes.and osteoblasts Conclusions The method of isolation BM-MSCs from tupaia by combination of gradient centrifugation and adherence culture is simple and feasible, BM-MSCs have differentiation potential into adipocytes and osteoblasts. |
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| Keywords: | Tupaia Bone marrow mesenchymal stem cells Cell culture Adipogenic induction Osteogenic induction |
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