颈动脉球囊损伤对高同型半胱氨酸诱导兔动脉粥样硬化病变的形成无明显影响

目的观察颈动脉球囊损伤对高同型半胱氨酸诱导的家兔动脉粥样硬化(atherosclerosis,As)病变形成的影响。方法新西兰家兔20只,随机分成对照组(n=10),As模型组(n=10)。两组均行颈动脉球囊拉伤术,术后As模型组每日经皮下注射甲硫氨酸80 mg/(kg/d)以诱导家兔形成动脉粥样硬化病变;对照组每日经皮下注射生理盐水。24周后处死动物,采用酶联免疫吸附法(ELISA)检测两组家兔实验前后血清同型半胱氨酸(Hcy)水平,运用光学显微镜检测24周后HE染色的动脉壁横切面病理改变,通过图像分析软件测量血管壁厚度变化,并与对照组进行比较。结果 24周后,As模型组家兔血清同型半胱氨酸含量较对照组明显升高(P0.05);同时主动脉壁出现不同程度地病理改变。但球囊损伤后的颈动脉壁厚度与对照组相比无统计学差异。结论球囊拉伤颈动脉对高同型半胱氨酸诱导的家兔动脉粥样硬化病变形成无显著影响。

Balloon injury does not significantly influence on the formation of atherosclerotic lesions induced by hyperhomocysteinemia in rabbits

Objective To explore the influence of carotid artery balloon injury on the formation of atherosclerotic lesions induced by hyperhomocysteinemia in rabbits. Methods Twenty New Zealand rabbits were divided into control group and model group (n=10) randomly. The left carotid arteries were injured by balloon catheterization in all rabbits. After operation, the model group was given methionine 80 mg/kg per day by subcutaneous injection for 24 weeks to induce atherosclerotic lesions, and the control group was given the same amount of normal saline. All rabbits were killed at the 24th week. ELISA was used to detect the content of homocysteine and HE staining was used to observe the pathological changes in the arterial wall. The arterial wall thickness was measured using an image analysis software. Results After 24 weeks, the level of serum homocysteine of the model group was significantly increased and pathological changes of aortic wall were observed in different degrees compared with that of the control group (P<0.05). But the carotid artery wall thickness of model group after balloon injury had no visible changes compared with that of the control group. Conclusion Balloon injury of carotid artery has no significant effect on the formation of atherosclerotic lesions induced by hyperhomocysteinemia.