HGF经HGF/Akt通路抑制肝癌细胞HCCC-9810的增殖

目的:观察肝细胞生长因子(HGF)对人胆管细胞型肝癌细胞HCCC-9810增殖的影响,并探讨其分子机制?方法:HGF处理HCCC-9810细胞后,用MTT及EdU法检测癌细胞的增殖;siRNA干扰技术抑制HCCC-9810细胞内源性Akt的表达,Western blot检测癌细胞Cyclin D1蛋白表达和Akt磷酸化的变化?结果:50和100 ng/ml HGF显著抑制肝癌细胞HCCC-9810的增殖?Western blot结果显示,HGF处理后的肝癌细胞Akt磷酸化和Cyclin D1表达量明显下调?siRNA干扰Akt的表达,阻断了HGF诱导的Cyclin D1表达量的下调及细胞增殖抑制?结论:HGF通过下调Akt磷酸化和Cyclin D1表达量抑制肝癌细胞HCCC-9810的增殖?

HGF inhibited the proliferation of liver carcinoma cell line HCCC-9810 via HGF/Akt signaling pathway

Objective:To identify the novel function of hepatocyte growth factor (HGF) in liver carcinoma cell HCCC-9810 proliferation and the underlying molecular mechanism. Methods:The proliferation of HCCC-9810 treated with HGF was detected by MTT assay and EdU assay. The expression of Akt in HCCC-9810 cells was inhibited by siRNA. Western blot was used to detect the expression of cyclin D1 and the phosphorylation level of Akt. Results:Both 50 ng/ml and 100 ng/ml HGF significantly inhibited the proliferation of HCCC-9810. Western blotting assay showed that HGF downregulated the expression of cyclin D1 and the phosphorylation level of Akt in HCCC-9810 cells. Conclusion:These results suggest that HGF inhibited the proliferation of HCCC-9810 by suppressing the expression of cyclin D1 and the phosphorylation level of Akt. SiRNA interfered the expression of Akt,and blocked HGF-induced downregulation of cyclin D1 expression and inhibition of cell proliferation.