戊巴比妥钠诱导的亚低温对雄性BALB/C小鼠血液学指标的影响

目的观察戊巴比妥钠诱导的亚低温对雄性BALB/C小鼠血液学指标的影响。方法将健康雄性BALB/C小鼠随机分为对照组(C)和亚低温组(M);亚低温组用戊巴比妥钠(100 mg/kg)腹腔注射诱导雄性BALB/C小鼠体温下降至28℃~30℃,维持4 h后自然复温,动物亚低温干预后2 h、24 h和72 h测定其肛温并取血测定血凝、电解质和血液细胞学指标;对照组给予等容体积的生理盐水,保持常温。结果本方法诱导的亚低温对雄性BALB/C小鼠体温和血凝三项与对照组相比差异无统计学意义(P﹥0.05),K+和Na+浓度高于对照组(P﹤0.01),外周血WBC低于对照组(P﹤0.01或P﹤0.05),白细胞分类比值发生变化,RBC、HGB、MCH、MCHC一过性降低(P﹤0.01或P﹤0.05)。结论戊巴比妥钠诱导的亚低温可对雄性BALB/C小鼠血液学指标产生影响。

Effects of mild hypothermia induced by pentobarbital sodium on hematology in male BALB/C mice

Objective To observe the effects of mild hypothermia induced by pentobarbital sodium on hematology in male BALB/C mice.Method Healthy male BALB/C mice were divided randomly into two groups：control group （ C） and mild hypothermia group（M）.The body temperature of the mild hypothermia group was maintained between 28℃ to 30℃（ anal temperature ） for 4 hours induced by pentobarbital sodium injected intraperitoneally , then recover unaffected . Anal temperature, coagulation, electrolytes, and blood cell indexes were examined in 2, 24, 72 hours after treated by mild hypothermia;Control group was given equal volume of saline volume at constant temperature .Results The body temperature and coagulation in mild hypothermia group showed no significant difference compared with the control group （ P﹥0.05）,but the concentration of K ＋and Na ＋in mild hypothermia group were higher than control group （P﹤0.01）, the number of WBC in mild hypothermia group was lower than control group （ P﹤0.01或P﹤0.05 ） , and the RBC、HGB、MCH、MCHC in mild hypothermia group were lower than control group transiently （P﹤0.01或P﹤0.05）.Conclusion Mild hypothermia induced by pentobarbital sodium affects some of hematological values in mice considerably .