一种皮肤致敏体外替代方法介绍-人细胞系激活试验

过敏性皮炎是由过敏原引起的皮肤病,主要是指人体接触到某些过敏原而引起皮肤红肿、发痒、脱皮等皮肤病症。目前,化学物致敏性评价主要依赖于动物实验。近年来,在动物福利和政府监管的推动下,采用体外培养细胞代替动物模型进行化学物致敏性的研究已成为趋势。人细胞系激活试验主要通过检测人THP-1细胞接触化学物后细胞表面标志物以及信号通路的变化判断其是否具有致敏性。该试验方法已被欧美和日本的多个实验室验证其可行性,但目前其临床应用价值还未被完全认可。随着研究的逐步深入,新的标志物和评定方法也在不断被开发和验证。

An alternative in vitro skin sensitization method -The human Cell line Activation Test

Allergic dermatitis is the skin diseases caused by allergen, is mainly refers to skin irritation, itching and peeling after exposure to certain allergens. At present, the sensitizing potential of chemicals is usually identified and characterized using animal test methods. Due to the increasing public and political concerns regarding the use of animals for the screening of new chemicals, using in vitro cell lines instead of animal models has become a trend. The human cell line activation test is mainly by detecting changes in cell surface markers and in the signal molecules after exposure to chemicals. The issue has been verified its feasibility by many laboratories in Europe, America and Japan. But at present the clinical value has not yet been approved. After some further optimization, new markers and methods are being developed and validated.