| 树鼩巴尔通体PCR方法的建立及初步应用 |
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| 引用本文： | 冯育芳,岳秉飞.树鼩巴尔通体PCR方法的建立及初步应用[J].中国比较医学杂志,2014,24(6):69-72. |
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| 作者姓名： | 冯育芳 岳秉飞 |
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| 作者单位： | 中国食品药品检定研究院实验动物资源研究所, 北京 100050;中国食品药品检定研究院实验动物资源研究所, 北京 100050;中国食品药品检定研究院实验动物资源研究所, 北京 100050;中国食品药品检定研究院实验动物资源研究所, 北京 100050;中国食品药品检定研究院实验动物资源研究所, 北京 100050;中国食品药品检定研究院实验动物资源研究所, 北京 100050 |
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| 基金项目： | 实验动物新品种的种群建立与质量标准化研究（2011BAI15B01）。 |
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| 摘 要： | 目的建立有效的树鼩巴尔通体PCR检测方法,并进行初步应用。方法针对NCBI公布的巴尔通体序列设计3对引物,通过对我国主要流行菌株的扩增实验来确定1对引物,并进行体系优化、特异性和敏感性测试;运用该PCR方法对60份树鼩样品进行检测,阳性样本进行序列测定。结果所建树鼩巴尔通体PCR检测方法特异性良好,灵敏度较高(2.0×10-5μg/mL),60份树鼩样本中有15份阳性样本,通过序列测定证实树鼩巴尔通体感染率为25%,与扩增结果完全相符,验证了该方法的可行性。结论该方法的建立为树鼩巴尔通体的检测奠定了基础。
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| 关 键 词： | 树鼩 巴尔通体 检测 PCR 应用 |
| 收稿时间： | 2014/2/26 0:00:00 |
| 修稿时间： | 2014/2/27 0:00:00 |
Establishment and Application of PCR Methods for Bartonella Detection in Tree shrew |
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| fengyufang and yuebingfei.Establishment and Application of PCR Methods for Bartonella Detection in Tree shrew[J].Chinese Journal of Comparative Medicine,2014,24(6):69-72. |
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| Authors: | fengyufang and yuebingfei |
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| Institution: | Institute for Laboratory Animal Resources, National Institutes for Food and Drug Control, Beijing 100050, China;Institute for Laboratory Animal Resources, National Institutes for Food and Drug Control, Beijing 100050, China;Institute for Laboratory Animal Resources, National Institutes for Food and Drug Control, Beijing 100050, China;Institute for Laboratory Animal Resources, National Institutes for Food and Drug Control, Beijing 100050, China;Institute for Laboratory Animal Resources, National Institutes for Food and Drug Control, Beijing 100050, China;Institute for Laboratory Animal Resources, National Institutes for Food and Drug Control, Beijing 100050, China |
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| Abstract: | Objective To establish an effective PCR assay for detection of Bartonella,and application of this assay in tree shrew. Methods Sequence of Bartonella was obtained from NCBI Genbank. Three pairs of primers were designed based on this sequence. One pair of primers was determined through amplifying the major strains in China. Sixty tree shrew blood samples were tested with this PCR assay. The positive amplified fragments were sequenced to verify the reliability of this method. Results A PCR method for detection of Bartonella is successfully established,with a high specificity and the sensitivity was of 2. 0 × 10- 5μg/mL. Among the tested 60 blood samples,15 positive cases were detected. Sequencing of the samples confirmed a 25% infection rate of Bartonella in the tree shrews,well consistent with the amplification results,and verified the applicability of this detection method. Conclusion The establishment of this method provides the basis for detection of Bartonella in tree shrew. |
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| Keywords: | Tree shrew Bartonella detection PCR Application |
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