IL-33转基因小鼠骨髓造血干/祖细胞向髓系细胞分化的能力增强

目的利用IL-33转基因小鼠研究IL-33对造血干/祖细胞的增殖和分化影响。方法利用流式细胞仪分析IL-33转基因小鼠及同窝野生对照小鼠的外周血、脾脏、骨髓细胞的免疫表型及造血干细胞分化不同阶段细胞的数量变化;利用体外成克隆实验和细胞周期分析研究IL-33对于造血干细胞增殖能力的影响。结果与野生型小鼠相比,IL-33转基因小鼠B细胞和T细胞在外周血中都明显降低,粒细胞在外周血和骨髓中都有明显增加;IL-33转基因小鼠的骨髓造血干细胞和多能祖细胞数量减少,共同淋系祖细胞数量减少,共同髓系祖细胞和粒单系祖细胞数量增加;IL-33转基因小鼠的造血干细胞处于S-G2-M的细胞增多;体外单克隆实验发现IL-33转基因小鼠造血干细胞形成的集落数增加。结论 IL-33转基因小鼠造血干细胞增殖能力增强,更易向髓系细胞分化。

IL-33 transgenic mice increase the myeloid differentiation in hematopoietic stem cells

Objective To study the influence of IL-33 on the Hematopoietic stem cells and progenitor cells. Methods Cells from the peripheral blood, spleen, thymus and bone marrow were stained with indicated antibodies and analyzed by flow cytometry. The LT-HSCs were sorted and culture using in vitro clonogenic assay. Results The percentage of B cells and T cells was decreased and the percentage of M cells was increased in the peripheral blood from IL-33 transgenic mice. Compared with the wildtype mice, the number of HSCs, MPPs and CLP was decreased; meanwhile the number of CMP and GMP was increased in the bone marrow from IL-33 transgenic mice. An in vitro clonogenic assay showed that LT-HSCs increased the ability to self-renew from IL-33 transgenic mice. And the percentage of S-G2-M stage hematopoietic stem cell was increased from IL-33 transgenic mice. Conclusion IL-33 increase the myeloid differentiation in hematopoietic stem cells.