| PTEN基因调控口腔鳞癌细胞TCA-8113增殖及凋亡 |
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| 引用本文： | 杨雨晗,邓缅.PTEN基因调控口腔鳞癌细胞TCA-8113增殖及凋亡[J].第三军医大学学报,2011,33(14):1484-1487. |
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| 作者姓名： | 杨雨晗 邓缅 |
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| 作者单位： | 1. 成都医学院生物医学系,细胞生物学和遗传学教研室,成都,610083
2. 成都医学院生物医学系,生物化学与分子生物学教研室,成都,610083 |
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| 基金项目： | 四川省教育厅科研基金(10ZC090)~~ |
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| 摘 要： | 目的 评估PTEN基因在人口腔鳞癌细胞系TCA-8113中的增殖抑制和促凋亡功能.方法 利用PTEN真核表达质粒转染TCA-8113细胞,另设PBS处理作为空白组,转染pcDNA3.1质粒和Lipofectamine处理作为对照组.采用MTT和克隆形成实验检测细胞增殖情况,采用流式细胞术、TUNEL和Western b...
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| 关 键 词： | 口腔鳞癌 PTEN 增殖 凋亡 |
PTEN gene expression inhibits proliferation and promotes apoptosis in human oral squamous carcinoma cell line TCA-8113 |
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| Yang Yuhan,Deng Mian.PTEN gene expression inhibits proliferation and promotes apoptosis in human oral squamous carcinoma cell line TCA-8113[J].Acta Academiae Medicinae Militaris Tertiae,2011,33(14):1484-1487. |
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| Authors: | Yang Yuhan Deng Mian |
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| Institution: | Yang Yuhan1,Deng Mian2(1Department of Cell Biology and Genetics,2Department of Biochemistry and Molecular Biology,School of Biomedical Sciences,Chengdu Medical College,Chengdu,Sichuan Province,610083,China) |
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| Abstract: | Objective To evaluate the effects of PTEN gene expression on inhibiting the proliferation and promoting the apoptosis of human oral squamous carcinoma cell line TCA-8113.Methods TCA-8113 cells were transfected with eukaryotic expression plasmid pcDNA3.1-PTEN to obtain an experimental group.Besides,TCA-8113 cells were also treated with PBS,plasmid pcDNA3.1 and Lipofectamine to obtain a blank group and two control groups(a pcDNA3.1-transfected group and a Lipofectamine-treated group).The proliferation of TCA-... |
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| Keywords: | oral squamous carcinoma PTEN proliferation apoptosis |
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