| shRNA抑制p115表达对胃癌体外血管形成的影响 |
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| 引用本文： | 文雪,易永芬,邓玮,屈玉玲,闫田静. shRNA抑制p115表达对胃癌体外血管形成的影响[J]. 第三军医大学学报, 2011, 33(16) |
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| 作者姓名： | 文雪 易永芬 邓玮 屈玉玲 闫田静 |
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| 作者单位： | 400016重庆,重庆医科大学基础医学院病理学教研室,分子医学与肿瘤研究中心 |
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| 基金项目： | 国家自然科学基金面上项目(30672431)~~ |
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| 摘 要： | 目的初步探讨胃癌BGC-823细胞高尔基体囊泡转运蛋白(golgi-vesicular transport protein,p115)对胃癌血管形成的影响及其可能机制。方法采用脂质体介导法将p115 shRNA-1318转染入胃癌BGC-823细胞株,经G418筛选出稳定转染的细胞株。利用Western blot、RT-PCR和细胞免疫荧光方法检测转染后的p115抑制效应及其对MIF、p-Akt、VEGF-A的调控情况;采用细胞活性检测实验、Transwell体外侵袭实验和血管形成实验方法分别检测转染p115 shRNA的胃癌BGC-823细胞对人脐静脉血管内皮细胞(human umbilical vein endothelial cells,HUVECs)增殖,迁移和血管形成的影响。结果 p115 shRNA-1318转染后胃癌BGC-823细胞p115、MIF、p-Akt和VEGF-A的蛋白及mRNA表达明显抑制(P<0.01);细胞免疫荧光:与对照组相比,p115 shRNA组p115、MIF及VEGF-A的色泽暗红且抑制率分别为63%、65%、68%;细胞活性检测:与对照组相比,p115 shRNA组...
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| 关 键 词： | 高尔基体囊泡转运蛋白p115 巨噬细胞移动抑制因子 人脐静脉血管内皮细胞 血管生成 |
Influence of shRNA-mediated p115 gene silencing on angiogenesis in gastric cancer cells in vitro |
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| Wen Xue,Yi Yongfen,Deng Wei,Qu Yuling,Yan Tianjing. Influence of shRNA-mediated p115 gene silencing on angiogenesis in gastric cancer cells in vitro[J]. Acta Academiae Medicinae Militaris Tertiae, 2011, 33(16) |
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| Authors: | Wen Xue Yi Yongfen Deng Wei Qu Yuling Yan Tianjing |
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| Affiliation: | Wen Xue,Yi Yongfen,Deng Wei,Qu Yuling,Yan Tianjing(Department of Pathology,Molecular Medicine and Cancer Research Center,College of Basic Medical Sciences Chongqing Medical University,Chongqing,400016,China) |
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| Abstract: | Objective To investigate the influence of Golgi-vesicular transport protein(p115) in gastric cancer cell line BGC-823 on the angiogenesis of gastric cancer and its possible mechanism.Methods BGC-823 cells were divided into a blank control group,a negative control group,and a p115 shRNA-1318-transfected group.In the p115 shRNA-transfected group,p115 shRNA-1318 was transferred into BGC-823 cells by liposome-mediated transfection.The well-transfected BGC-823 cells were screened out using G418.Western blotting ... |
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| Keywords: | p115 MIF HUVECs angiogenesis gastric cancer |
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