婴幼儿咽喉乳头瘤组织人乳头瘤病毒感染的探讨

目的 探讨温州地区人乳头瘤病毒感染和婴幼儿咽喉乳头状瘤的关系。方法 应用聚合酶链反应和核酸斑点杂交技术检测３５例婴幼儿咽喉乳头瘤组织和１０例对照组组织（小儿声带小结）ＨＰＶ６、１１、１６、１８、３３５个型别的ＤＮＡ。结果 乳头瘤组织ＨＰＶ感染率为９１．４％（３０／３５），其中ＨＰＶ６型检出率为５４．２％（１９／３５），ＨＰＶ１１型感染率为２５．７％（９／３５），多重型别ＨＰＶ６＋１１感染率为１１．

The study of human papilloma viruses infection in juvenile pharynlaryngeal papillomatosis by PCR and dot blot hybridization]

OBJECTIVE: To study the relationship between infection of human papillomavirus and juvenile pharynlaryngeal papilloma. METHODS: Polymerase chain reaction (PCR) and dot blot hybridization were used to detect HPV6,11,16,18,33 DNA of 35 samples of pharynlaryngeal papilloma and 10 samples of vocal nodule. RESULTS: The positive rate of HPV in pharynlaryngeal papilloma was 91.4% (30/35). Among them the positive rates of HPV6 and HPV11 were 54.2%(19/35) and 25.7%(9/35), the positive rate of multiple types of HPV6 + 11 was 11.4%(4/35). The positive rate of HPV16,18,33 was negative. The positive rate of HPV in vocal nodule was negative and were significantly different from that of the pharynlaryngeal papilloma group. CONCLUSION: The results suggest that infection with HPV, especially with HPV6, is closely associated with the development of juvenile pharynlaryngeal papilloma in Wenzhou area. HPV-DNA detected by PCR and dot blot hybridization has highly specificity and sensitivity.