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Isoelectric focusing of herpes simplex virus
Authors:S. Olofsson
Affiliation:1. Department of Virology, Institute of Medical Microbiology, University of G?teborg, G?teborg, Sweden
Abstract:Herpes simplex virus was inserted into a preformed stable pH gradient and electrofocused for about one hour. Dextran was used as the density gradient forming agent. Virions banded at pH 4.9+/-0.1 and nucleocapsids at 4.1+/-0.05. About 10-20 per cent of infective virus was recovered.
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