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Distribution of hemagglutinin and neuraminidase on influenza virions as revealed by immunoelectron microscopy
Authors:K G Murti  R G Webster
Affiliation:1. Emergency Centre for Transboundary Animal Diseases – India, Food and Agriculture Organization of the United Nations, Animal Quarantine & Certification Service Station Kapashera, New Delhi 110037, India;2. Division of Pathway Medicine, School of Biomedical Sciences, College of Medicine and Veterinary Medicine, Chancellor’s Building, 49 Little France Crescent, Edinburgh EH16 4SB, United Kingdom;3. Emergency Centre for Transboundary Animal Diseases (ECTAD)/Regional Support Unit for SAARC Countries, Food and Agriculture Organization of the United Nations, KSK Building, Block B, Third Floor, Pulchowk, Kathmandu, Nepal;4. Emergency Center for Transboundary Animal Diseases (ECTAD), FAO Regional Office for Asia and the Pacific (FAO-RAP), 39 Phra Atit Road, Bangkok 10200, Thailand;5. Veterinary Clinical Sciences, Royal (Dick) School of Veterinary Studies, College of Medicine and Veterinary Medicine, University of Edinburgh, Easter Bush Veterinary Centre Roslin, Midlothian EH25 9RG, United Kingdom;6. Biological Control and Spatial Ecology, CP160/12 Université Libre de Bruxelles, Avenue FD Roosevelt 50, B-1050 Brussels, Belgium;7. Fonds National de la Recherche Scientifique (F.R.S.-FNRS), rue d’Egmont 5, B-1000 Brussels, Belgium;1. Lab. d’Etude Physico-Chimique des matériaux et Application à l’Environnement, Faculté de Chimie, USTHB, BP 32 El Alia, Bab Ezzouar, 16111 Alger, Algérie;2. ENSSMAL, BP 19 Bois des Cars, Dely Ibrahim, 16320 Alger, Algérie;3. Centre de Recherche Scientifique et Technique en Analyses Physico-Chimiques (C.R.A.P.C), BP 384, Bou-Ismail, RP, 42004 Tipaza, Algérie;4. Adscientis, 1 Rue Kastler, 68310 Wittelsheim, France;5. Université de Toulouse, Mines Albi, CNRS UMR 5302, Centre RAPSODEE, Campus Jarlard, F-81013, Albi Cedex 09, France;1. Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, Liaoning, 110016, China;2. Department of Plant and Soil Sciences, University of Delaware, Newark, DE, 19716, USA;3. Delaware Biotechnology Institute, University of Delaware, Newark, DE, 19716, USA;4. Department of Animal and Food Sciences, University of Delaware, Newark, DE, 19716, USA;5. University of Chinese Academy of Sciences, Beijing, 100039, China;6. Department of Biosystems Engineering and Soil Science, University of Tennessee, Knoxville, TN, 37996, USA;1. Department of Animal Development and Physiology, Kyoto University, Yoshida-Konoe, Sakyo, Kyoto 606-8501, Japan;2. Responses to Environmental Signals and Stresses, Graduate School of Biostudies, Kyoto University, Yoshida-Konoe, Sakyo, Kyoto, Kyoto 606-8501, Japan;3. Japan Science and Technology Agency, Core Research for Evolutional Science and Technology (CREST), Tokyo 102-0081, Japan;1. The Ministry of Education Key Laboratory of Protein Science, Beijing Advanced Innovation Center for Structural Biology, Collaborative Innovation Center for Biotherapy, School of Life Sciences, Tsinghua University, Beijing 100084, China;2. Comprehensive AIDS Research Center, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Department of Basic Medical Sciences, School of Medicine, Tsinghua University, Beijing 100084, China;3. Molecular Biophysics and Integrated Bioimaging, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA;4. Department of Microbiology and Immunology, National Cheng Kung University Medical College, Tainan 701, Taiwan;5. Collaborative Innovation Center for Biotherapy, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu, China
Abstract:Monoclonal antibodies specific for the hemagglutinin (HA) or the neuraminidase (NA) of influenza viruses were used in immunoelectron microscopic studies to determine the distribution of the two surface spikes on the virion. Indirect immunogold staining revealed that the HA is uniformly distributed on the virion while the NA occurs in discrete areas. Crosslinking and low temperature studies argue against redistribution of the HA and NA after antibody attachment and indicate that the NA on influenza virus occurs in patches.
Keywords:
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