三磷酸肌醇和bax基因表达变化在genistein抑制裸鼠肝癌增长中的作用

目的: 探讨三磷酸肌醇( IP3) 和Bax基因表达变化在genistein治疗裸鼠移植人肝癌中的作用。方法:以裸鼠移植人肝癌为对照, 对照组腹腔注入含0.04%DMSO的RPMI1640培养基0.05ml/g，Genistein治疗组腹腔注入genistein 1mg/kg/d，3周后观察肝癌增长情况，并应用同位素试剂盒检测肝癌组织IP3 含量,RT-PCR分析癌组织Bax mRNA表达，Western blotting 分析肝癌组织不bax蛋白表达。结果: 治疗组肝癌体积和重量均显著低于对照组体积(12.6±11.6) mm3 vs (52.3±26.5) mm3，重量(42.7±27.8) mg vs (91.3±31.4) mg）. P<0.01]，IP3 含量显著低于对照组（13.4±1.4）pmol/mg protein vs （35.3±6.6）pmol/mg protein. P < 0.01]，bax mRNA表达显著高于对照组RI（灰度与面积之积的相对强度）0.88±0.2 vs 0.56±0.15. P < 0.05]，bax蛋白表达显著高于对照组RI（灰度与面积之积的相对强度）2.86±0.80 vs 1.37±0.48. P < 0.05]。结论: Genistein能减少IP3 生成,上调肝癌组织bax基因表达,抑制裸鼠移植人肝癌增长。

The Role of Trisphosphate Inositol and Bax gene expression in inhabiting hepatocellular carcinoma of nude mice by genistein

ABSTRACT] 　AIM: To probe into the role of 1 , 4 , 5 - trisphosphate inositol ( IP3) and bax gene expression in inhabiting hepatocellular carcinoma of nude mice by genistein METHODS: animals with hepatocellular carcinoma were treated with genistein 1mg/kg/d (ip)for 3 weeks, the volume and weight of tumaor was measured,IP3 , Bax mRNA, Bax protein were assayed by IP3 - 3H] Birtrak assay ,RT-PCR, Western blotting, respectively. RESULTS: The tumor volume and weight of animals treated with genistein was lower than control(12.6±11.6) mm3 vs (52.3±26.5) mm3, (42.7±27.8) mg vs (91.3±31.4) mg). P<0.01]，IP3 continent was lower than that of control（13.4±1.4）pmol/mg protein vs （35.3±6.6）pmol/mg protein. P<0.01]，bax mRNA expression was higher in group treated with genistein than that of controlRI which was the gray degree multiply area of bax / the gray degree multiply area of β- actin 0.88±0.2 vs 0.56±0.15. P<0.05]，bax mRNA expression was higher in group treated with genistein than that of control(2.86±0.80 vs 1.37±0.48. P<0.05). CONCLUSION: Genistein can inhabit growth of hepatocellular carcinoma tansplanted into liver of nude mice by reducing IP3 production and up-regulating bax gene expression.