KATP抑制剂对七氟醚诱导乳鼠心肌细胞HSP70表达的影响

目的探讨HSP70在七氟醚诱导乳鼠心肌细胞预适应中的作用及其内在联系. 方法第2代培养心肌细胞随机分为正常对照组、缺氧/复氧组、七氟醚组、优降糖组、优降糖+七氟醚组,每组均缺氧2 h,复氧48 h.分别取复氧0、1、12、24、36和48 h的细胞用免疫组化染色检测乳鼠心肌细胞HSP70的表达.结果在各个时点,七氟醚组的HSP70表达最强,显著高于正常对照组、缺氧/复氧组、优降糖组和优降糖+七氟醚组(P<0.01),随着复氧时间的延长,七氟醚组HSP70表达从1 h开始增加,24 h表达最强, 具有显著统计学意义(P<0.05).且优降糖+七氟醚组HSP70表达与正常对照组、缺氧/ 复氧组、优降糖组组间无显著性差异(P>0.05).结论 HSP70和K ATP 通道均参与了七氟醚诱导乳鼠心肌细胞产生预适应过程,阻止KATP通道则抑制HSP70 的表达.

Influence of KATP Channel Inhibitor on the Changes of HSP70 Expression in Sevoflurane-induced Neonatal Rat Cardiomyocytes

Objective To study the roles of K ATP channel and HSP70 in sevoflurane induced preconditioning in neonatal rat cardiomyocytes and their mutual relationship. Methods The second generation of primary cultured cardiomyocytes were randomly divided into 5 groups: normal control, anoxia/reoxygenation, sevoflurane preconditioning, glyburide and glyburide plus sevoflurane. In each group, the cardiomyocytes were exposed to a 2 hour anoxia, followed by a 48 hour reoxygenation. We detected HSP70 expression at 0, 1, 12, 24, 36 and 48 hours after reoxygenation respectively. Results At each time point of reoxygenation, the expression of HSP70 in sevoflurane preconditioning group was significantly higher than that of normal control, anoxia/reoxygenation, glyburide and glyburide plus sevoflurane groups ( P <0.01). There was no significant difference concerning HSP70 expression among normal control, anoxia/reoxygenation, glyburide and glyburide plus sevoflurane groups( P >0 05). Conclusion Both HSP70 and K ATP channel may be involved in the process of sevoflurane preconditioning in neonatal rat cardiomyocytes. Blocking the K ATP channel can inhibit the expression of HSP70.