Increasing the denaturation temperature during the first cycles of amplification reduced allele dropout from single cells for preimplantation genetic diagnosis |
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| Authors: | Ray Pierre F. |
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| Affiliation: | Human Embryology Laboratory, Institute of Obstetrics and Gynaecology, Royal postgraduate Medical School, Hammersmith Hospital Du Cane Road, London W12 0NN, UK |
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| Abstract: | Single cell polymerase chain reaction (PCR) for preimplantationgenetic diagnosis (PGD) needs to be highly efficient and accurate.In some single cells from human embryos presumed to be heterozygousfor the |
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