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西藏地区鼠疫耶尔森菌基因分型研究
引用本文:戴瑞霞,戴二黑,周冬生,赵海红,李存香,金丽霞,杨晓艳,祁美英,崔百忠,李敏,杨瑞馥,西绕若登,次仁顿珠. 西藏地区鼠疫耶尔森菌基因分型研究[J]. 中国地方病学杂志, 2007, 26(2): 159-160
作者姓名:戴瑞霞  戴二黑  周冬生  赵海红  李存香  金丽霞  杨晓艳  祁美英  崔百忠  李敏  杨瑞馥  西绕若登  次仁顿珠
作者单位:1. 811602,西宁,青海省地方病预防控制所鼠疫防治科
2. 军事医学科学院微生物流行病学研究所
3. 西藏自治区疾病预防控制中心
摘    要:目的研究西藏地区鼠疫菌的基因组成和基因分型。方法对西藏地区分离到的75株鼠疫菌,按照周东生等已经证实的22个差异区段(DFRs)设计引物,对每株鼠疫菌的每个基因差异区段都采用聚合酶链反应(PCR)进行验证。结果西藏地区75株鼠疫菌共包括3个基因型,即5、6、10型。藏北地区分离的鼠疫菌为5型和6型,分别占24.0%、13.3%;藏南地区分离的鼠疫菌为10型,占62.7%。结论在西藏地区鼠疫菌中,青藏高原型鼠疫菌为5和6型,岗底斯山型鼠疫菌为10型。

关 键 词:耶尔森菌 鼠疫 聚合酶链反应 基因型 差异区段
修稿时间:2006-03-20

Genomic typing of strains of Yerinia pestis in Tibet Region
DAI Rui-xia,DAI Er-hei,ZHOU Dong-sheng,ZHAO Hai-hong,LI Cun-xiang,JIN Li-xia,YANG Xiao-yan,QI Mei-ying,CUI Bai-zhong,LI Min,YANG Rui-fu,XI-RAO Ruo-deng,CI-REN Dun-zhu. Genomic typing of strains of Yerinia pestis in Tibet Region[J]. Chinese Jouranl of Endemiology, 2007, 26(2): 159-160
Authors:DAI Rui-xia  DAI Er-hei  ZHOU Dong-sheng  ZHAO Hai-hong  LI Cun-xiang  JIN Li-xia  YANG Xiao-yan  QI Mei-ying  CUI Bai-zhong  LI Min  YANG Rui-fu  XI-RAO Ruo-deng  CI-REN Dun-zhu
Affiliation:Institute for Endemic Disease Prevention and Control of Qinghai Province, Xining 811602, China
Abstract:Objective To study the distribution of genomovars and microevolution of Yersinia pestis(Y. pestis)in Tibet Region.Methods Primer pairs targeting the twenty-two different regions(DFRs)designed by ZHOU Dong-sheng were used to detect the presence or deletion of each DFR in 75 strains of Y.pestis isolated from Tibet Region.Results 75 strains of Y.pestis in Tibet Region were elassified as genomovar 5,6 and 10.Genomovar 5 and 6 strains of Y.pestis distributed in the north of Tibet.accounting for 24.0% and 13.3%,respectively.Genomovar 10 strains of Y.pestis distributed in the south of Tibet,accounting for 62.7%.Conclusion Strains of Y.pestis of Qing- Tibet Plateau are classified into genomovar 5 and 6,Gangdis Monutain strain is of genomovar 10.
Keywords:Yerinia pestis    Polymerase chain reaction    Genotype    Different region
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