| 干细胞因子对红白血病细胞系TF-1凋亡的抑制作用 |
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| 引用本文： | 王利红,胡志远,王嘉玺,贺福初. 干细胞因子对红白血病细胞系TF-1凋亡的抑制作用[J]. 军事医学科学院院刊, 2000, 24(1): 33-35 |
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| 作者姓名： | 王利红 胡志远 王嘉玺 贺福初 |
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| 作者单位： | 1. 军事医学科学院基础医学研究所,北京,100850
2. 军事医学科学院放射医学研究所,北京,100850 |
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| 基金项目： | 国家自然科学基金!重点项目资助课题 (39730 2 70 ) |
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| 摘 要： | 目的 :探讨干细胞因子 (stemcellfactor ,SCF)阻止红白血病细胞系TF 1进入凋亡程序的作用。方法 :进行3H TdR掺入实验、细胞存活实验、细胞基因组DNA电泳分析和流式细胞术分析。结果 :发现TF 1细胞对SCF的反应存在量 半高效关系 ,在SCF存在时细胞存活率明显提高 ,TF 1细胞系在SCF饥饿 2 4h时出现DNA梯型条带 ;流式细胞术检测显示 ,在SCF饥饿 36h后 ,在G1峰前出现凋亡特有的AP峰。结论 :SCF有阻滞或抑制红白血病细胞系TF 1凋亡的作用。
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| 关 键 词： | 干细胞因子 TF－1 细胞凋亡 红白血病 |
Inhibitory effect of stem cell factor on apoptosis of erythroleukemia cell line TF-1 |
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| WANG Li-hong,HU Zhi-yuan,WANG Jia-xi,HE Fu-chu. Inhibitory effect of stem cell factor on apoptosis of erythroleukemia cell line TF-1[J]. Bulletin of the Academy of Military Medical Sciences, 2000, 24(1): 33-35 |
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| Authors: | WANG Li-hong HU Zhi-yuan WANG Jia-xi HE Fu-chu |
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| Abstract: | Objective: To explore the blocking action of stem cell factor(SCF) on entrance of erythroleukemia(EL) cell line TF 1 into apoptosis. Methods: 3H TdR incorporation, cell survival test, electrophoresis of cell genomic DNA, and flow cytometry analysis were used to analyze apoptosis. Results: Correlation befween concentration and effect existed in response of TF 1 cells to SCF. In presence of SCF survival rate was significantly increased, and under SCF starvation TF 1 cells showed DNA pattern on agarose gel electrophoresis. After the starvation of SCF for 36 hours, the intrinsic AP peak of apoptosis appeared before G 1 peak. Conclusion: SCF possesses blocking action on the apoptosis of EL cell line TF 1. [ |
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| Keywords: | stem cell factor factor dependent cell line apoptosis |
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