海南海桑内生真菌Bionectria ochroleuca HHS111023次生代谢产物的研究

目的 研究中国特有红树植物海南海桑内生真菌Bionectria ochroleuca HHS111023的次生代谢产物及其生物活性。方法 利用多种柱色谱技术对次生代谢产物进行分离纯化；通过核磁与质谱数据分析结合理化常数及文献比对，鉴定化合物的结构；分别采用纸片琼脂扩散法和MTT法对化合物的抗菌活性和细胞毒活性进行测试。结果 从海南海桑内生真菌Bionectria ochroleuca HHS111023的次生代谢产物中分离鉴定了7个化合物：Lasiodiplodin (1)、(R)-de-O-methyllasiodiplodin (2)、(5S)-5-hydroxylasiodiplodin (3)、 (5R)-5-hydroxylasiodiplodin (4)、methyl (E)-11,12,15-trihydroxyoctadec-13-enoate (5)、对羟基苯乙醇 (6)、对羟基苯乙酸甲酯 (7)。化合物1和2分别对白色念珠菌和青枯雷尔氏菌具有抗菌活性，且分别对SGC-7901和K-562具有体外细胞毒活性。结论 化合物1 ~ 4为首次从淡色生赤壳菌Bionectria ochroleuca次生代谢产物中分离得到，化合物5为首次分离自微生物次生代谢产物；海南海桑内生真菌Bionectria ochroleuca HHS111023能产生具有抗菌和细胞毒活性的化合物。

Studies on the secondary metabolites from the endophytic fungus Bionectria ochroleuca HHS111023 of Sonneratia hainanensis

Objective To study the bioactive secondary metabolites from the endophytic fungus Bionectria ochroleuca HHS111023 of Sonneratia hainanensis, an endemic mangrove tree in China. Methods The isolation and purification of the compounds were performed via various chromatographic techniques, and their structureswere determined through spectroscopic analysis of NMR and ESI-MS combined with their physicochemical data.The antimicrobial and cytotoxicity activities of the compounds were evaluated by paper disco diffusion method and MTT method, respectively. Results Seven compounds were isolated from the fermentation broth of Bionectria ochroleuca HHS111023 and identified as Lasiodiplodin (1), (R)-de-O-methyllasiodiplodin (2), (5S)-5-hydroxylasiodiplodin (3), (5R)-5-hydroxylasiodiplodin (4), methyl (E)-11,12,15-trihydroxyoctadec-13- enoate (5), 4-hydroxyphenethyl alcohol (6) and p-hydroxyphenylacetic acid methyl ester (7). Compound 1 and 2 showed inhibitory effect against Candida albicans and Ralstonia solanacearum, respectively. Moreover, they also exhibited cytotoxic activities in vitro against SGC-7901 and K-562 tumor cell lines, respectively. Conclusion compounds 1?4 were isolated from Bionectria ochroleuca for the first time, and compound 5 was reported as the microbial metabolites for the first time. Furthermore, the endophytic fungus Bionectria ochroleuca HHS111023 of Sonneratia hainanensis could produce compounds with antimicrobial and cytotoxicity activities.