| 高压氧预处理对高原肺水肿大鼠肺水通道蛋白-5的影响 |
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| 引用本文： | 李茁,赵立明,王丛,刘雪华,吕春凤,高春锦. 高压氧预处理对高原肺水肿大鼠肺水通道蛋白-5的影响[J]. 武警医学, 2011, 22(11): 938-942 |
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| 作者姓名： | 李茁 赵立明 王丛 刘雪华 吕春凤 高春锦 |
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| 作者单位： | 首都医科大学附属北京朝阳医院高压氧科,100020;北京航天总医院,100076 |
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| 摘 要： | 目的 观察高压氧预处理( hyperbaric oxygen preconditioning,HBO -PC)对高原肺水肿(high - altiude pulmonary edema,HAPE)大鼠肺水通道蛋白-5(NQP5)的影响.方法 38只Wistar大鼠,分对照组、HAPE模型组(HAPE组)及高压氧预处理组(HBO组)制作HAPE模型后,分别以Western Blot法及Real - Time PCR法观察各组大鼠肺AQP5蛋白、基因表达,同时观察肺水含量及病理变化.结果 HAPE组肺湿/干比明显高于Control组；HBO组肺损伤积分明显低于HAPE组；HAPE组AQP5及AQP5mRNA较Control组显著降低,HBO组AQP5及AQP5mRNA均明显高于HAPE组；肺组织损伤与肺AQP5表达有密切的相关关系,且呈负相关 结论 AQP5表达降低可能参与了HAPE的发病机制；HBO - PC能上调AQP5表达,具有预防和减轻HAPE的作用
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| 关 键 词： | 高原肺水肿 高压氧预处理 水通道蛋白5 肺组织病理 肺湿/干比值 |
Effect of hyperbaric oxygen preconditioning on expression of lung aquaporin - 5 in rat models of high altitude pulmonary edema |
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| LI Zhuo,ZHAO Liming,WANG Cong,LIU Xuehua,LV Chunfeng,GAO Chunjin. Effect of hyperbaric oxygen preconditioning on expression of lung aquaporin - 5 in rat models of high altitude pulmonary edema[J]. Medical Journal of the Chinese People's Armed Police Forces, 2011, 22(11): 938-942 |
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| Authors: | LI Zhuo ZHAO Liming WANG Cong LIU Xuehua LV Chunfeng GAO Chunjin |
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| Affiliation: | LI Zhuo~1,ZHAO Liming~1,WANG Cong~1,LIU Xuehua~1,LV Chunfeng~2,and GAO Chunjin~1.1.Department of Hyperbaric Oxygen,Beijing Chaoyang Hospital,Capital Medical University,Beijing 100020,China,2.Beijing Aerospace Hospital,Beijing 100076 |
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| Abstract: | Objective To investigate the possibility of applying hyperbaric oxygen preconditioning(HBO-PC)to reduce or prevent high-altitude pulmonary edema(HAPE)and to find out if aquaporin-5(AQP5)is involved in HAPE pathogenesis using a rat model.Methods Rats were divided into three groups:control,HBO and HAPE.Western blotting and real-time PCR were used to analyze the expression of AQP5 in the lung.The wet-to-dry weight ratio(W/D weight ratio)and morphology of the lung were also examined.Results An increased lung W/... |
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| Keywords: | high-altitude pulmonary edema hyperbaric oxygen preconditioning Aquaporin 5 lung wet-to-dry weight ratio lung morphology |
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