高眼压模型大鼠血清中hsp27抗体的检测及其意义

 目的 观察高眼压模型大鼠血清中热休克蛋白27(heat shock protein 27,hsp27)抗体的情况,并分析眼压与hsp27抗体之间的关系.方法 将51只Wistar大鼠随机分为高眼压组(34只),Sham对照组(17只).采用双极水下电凝器电凝大鼠右眼巩膜表面3组静脉及角膜缘周围血管,升高大鼠眼内压,建立高眼压动物模型,分别于手术后1、2、4、6、8周处死大鼠,处死前测大鼠双眼眼压,并收集血清1ml供酶联免疫吸附实验(enzyme-linked immunosorbeat assay,ELISA)测定大鼠血清中hsp27抗体水平.结果 高眼压组右眼术后眼压明显升高.术前眼压(15.00±2.498)mmHg;术后3d眼压:(27.44±2.776)mmHg;术后2周眼压:33.96±2.603)mmHg;术后8周眼压:(35.50±2.390)mmHg.术后高眼压组右眼眼压与术前、左眼及Sham对照组右眼比较,差异有统计学意义(P＜0.05)；高眼压组不同时间点血清中hsp27抗体水平相比差异有统计学意义(P＜0.05).结论 随着眼压的升高以及高眼压持续时间的延长,大鼠血清中的hsp27抗体水平逐渐升高.

Study of heat shock protein 27 antibody in a rat model of high intraocular pressure

Objective To observe the activity of heat shock protein 27 antibody in blood serum in an experimental rat midel of high intraocular pressure and analyze the relation between intraocular pressure and heat shock protein 27 antibody in blood serum. Methods 51 Wistar rats were randomly divided into high intraocular pressure group(n=34),Sham control group(n=17).Intraocular pressure(IOP)was elevated by electrocoagulation of three episcleral and limbal veins in the right eye of rats.1 W,2W,4W,6W,8W after operation...