青藤碱对膝骨性关节炎兔关节液及血清白细胞介素1β含量的影响

目的：探讨关节腔内注射青藤碱对膝骨关节炎兔关节液及血清中白细胞介素1β（interleukin-1β，IL-1β）含量的影响。方法：除正常对照组（n=4）外，36只免采用左后肢膝关节伸直位石膏管型制动法建立兔骨关节炎模型。造模6周后青藤碱组兔关节腔内注射青藤碱，1次／N，连续5周，透明质酸组关节腔内注射透明质酸，模型组关节腔内注射生理盐水，正常组不治疗。分别于治疗前及治疗后采用酶联免疫吸附测定法检测关节液及血清IL-1β含量，并对软骨组织进行Mankin评分。结果：与模型组比较，青藤碱组骨性关节炎兔血清及关节液中IL-1β含量下降（P〈0．01），且青藤碱组低于透明质酸组（P〈0．05）。青藤碱组的软骨Mankin评分明显低于透明质酸组（P〈0．05）。结论：青藤碱可能通过抑制关节液及血清中IL—1β表达降低兔骨性关节炎模型的关节退变程度。

Effects of sinomenine on synovial fluid and serum content of interleukin- 1β in rabbits with osteoarthritis

Objective： To investigate the effects of intra-articular injection of sinomenine on interleukin-1β （IL-1β） content in synovial fluid and serum in rabbits with osteoarthritis （OA）. Methods： Degenerative osteoarthritis in the knee joint of left posterior limb was induced in 36 rabbits by full extension using plaster cast for 6 weeks. Then the rabbits were randomly divided into untreated group, hyaluronic acid group and sinomenine group. Another 4： normal rabbits were selected as normal control group. Rabbits in the sinomenine group and the hyaluronic acid group received intraarticular injections of sinomenine and hyaluronic acid once a week for 5 weeks, respectively. The content of IL-1β in synovial fluid and serum were measured before and after treatment by enzyme-linked immunosorbent assay. The pathological changes of cartilaginous tissue were analyzed by using Mankin＇s score. Results： Compared with those in the untreated group, synovial fluid and serum IL-1β contents in the sinomenine group and thehyaturonic acid group were significantly decreased （P〈0.0]）. And the synovial fluid and serum IL-1β contents in the sinomenine group were lower than those in the hyaluronic acid group （P〈0.05）. The mean Mankin＇s score of cartilage in the sinomenine group was significantly lower than that in hyaluronic acid group （P〈0.05）. Conclusion： Sinomenine may reduce the degree of articular degeneration in rabbit with OA through decreasing the content of IL-1β in synovial fluid and serum.