整合素连接激酶和β1整合素在大鼠烫伤创面愈合过程中表达的实验研究

目的探讨大鼠烫伤创面愈合过程中整合素连接激酶（ILK）和β1整合素的表达以及在创面愈合中的作用。方法健康雌性SD大鼠18只,随机选取其中15只作为实验组,建立深Ⅱ度烫伤模型,另外3只为对照组,不做处理。实验组分别于烫伤模型建立后第1、5、10、15、20天随机选取3只大鼠,切取背部两侧全层创面,运用免疫组织化学的方法检测创面中ILK、β1整合素的表达。对照组取背部正常皮肤作为对照。结果实验组烫伤后第5、10、15天大鼠烫伤创面中ILK的表达明显高于对照组和实验组烫伤后第20天（P〈0.05）;创面中β1整合素的表达在烫伤后第5天明显高于对照组（P〈0.05）,实验组烫伤后第10、15天则明显高于对照组和实验组烫伤后第20天（P〈0.05）。结论ILK、β1整合素在大鼠创面愈合过程中表达明显增加,ILK可能通过参与整合素信号通路,在调节创面细胞的增生以及细胞与ECM相互作用中扮演着重要的角色。

Experimental study on the expression of integrin-linked kinase and beta-1 integrin during burned wound healing in rats

Objective To investigate the expression of integrin-linked kinase and beta-1 integrin, as well as their e ffects, during burned wound healing in rats.Methods Fifteen SD rats were prepared as burns models with deep partial thickness burned wounds by scalding.Three rats were selected to detect the expression of integrin-linked kinase and beta-1 integrin at random in day 1,day 5,day 10,day 15 and day 20 by immunohistochemistry respectively.Normal skin from 3 SD rats was as control.Results The expression of beta-1 integrin was significantlyhigher in day 5, day 10 and day 15 than in normal skins and in the healed wounds.in the other hand, the level of beta-1 integrin was significantly increased in day 10 and day 15, compared with normal skins and healed wounds.Conclusions The expression of integrin-linked kinase and beta-1 integrin was significantly increased in the course of wound healing.ILK possibly plays an important role in the regulation of wound cell proliferation and cell interaction with extracellular matrixviaintegrin signaling pathway.