胶质细胞源性神经营养因子对脊髓前角神经元的保护作用

目的 探讨胶质细胞源性神经营养因子（ＧＤＮＦ）对坐骨神经切断后引起的脊髓前角运动神经元退行性变的影响。方法 以硅胶管套接切断的大鼠坐骨神经为实验模型，２４只大鼠随机分为２组，治疗组硅胶管内注射ＧＤＮＦ，对照组注射等渗盐水，术后２周观察大鼠后肢运动功能，并进行脊髓切片的尼氏染色、酸性磷酸酶（ＡＣＰ）组织化学染色及原位末端标记（ＴＵＮＥＬ）法染色。结果 与对照组相比，治疗组后肢运动功能明显改善；脊髓前

Protective effect of glial cell line-derived neurotrophic factor on spinal motoneurons after sciatic nerve injury in rats

Objective To investigate the effect of glial cell line derived neurotrophic factor(GDNF) on spinal motoneurons following sciatic nerve axotomy. Methods Rats with the right sciatic nerve transected were used as models. Twenty four rats were randomized into a treated group (GDNF was injected into the silica-gel tube) and a control group (Isotonic saline was injected). After silicone tubulization of sciatic nerve in adult rats, normal saline was injected into silicone chamber of the control group, and GDNF solution was injected in the treated group during operation. Two weeks after operation, hindlimb motor functions were measured and spinal sections were stained by Nissl, acid phosphatase(ACP) enzyme histochemistry and TUNEL methods. Results Compared with the control group, the hindlimb motor function was improved, the percentage of the survived spinal motoneuron was increased from 85.3% to 95.8%( P <0.01), the percentage of AC P stained area was significantly decreased from 376% to 252%( P <0.01)and TUNEL positive cells were reduced from 34.8 to 13.2 ( P <0.01)in the treated group. Conclusions GDNF could protect the degenerated spinal motoneuron after sciatic nerve axotomy.