替吉奥对人肺癌细胞A549及胃癌细胞SGC-7901的体外抑制作用

目的研究替吉奥对人肺癌细胞A549及胃癌细胞SGC-7901的体外抑制作用。方法采用四甲基偶氮唑盐（MTT）测定不同浓度的替吉奥对A549细胞及SGC-7901细胞的抑制作用，并选择5-氟尿嘧啶（5-Fu）作对比，计算半数抑制浓度（IC50）。运用流式细胞仪观察替吉奥对A549及SGC-7901细胞周期和凋亡的影响。结果替吉奥对2种细胞的抑制作用随浓度的增加而增加，呈明显浓度依赖性。替吉奥对3．549细胞及SGC-7901细胞的IC50分别为78．33μg／mL和63．40μg／mL，明显低于5-Fu的241．60μg／mL和201．68μg／mL。替吉奥能明显使2种细胞的细胞周期被阻滞于G0／G1及S期，且对A549细胞及SGC-7901细胞的凋亡率分别为（36．64±3．92）％和（38．69±3．02）％，明显高于空白组。结论替吉奥对人肺癌细胞A549及胃癌细胞SGC-7901的体外抑制作用明显，显著优于5-Fu。

Inhibitory effects of S-1 on human lung cancer cell A549 and gastric cancer cell SGC-7901 in vitro

Objective To study the inhibitory effects of S-1 on human lung cancer cell A549 and gastric cancer cell SGC-7901 in vitro. Methods The inhibitory effects of different concentrations of S-1 on A549 and SGC-7901 cells were detected by methyl thiazolyl tetrazolium （MTT） , and 5-flu- orouracil （5-Fu） was used as a control. Besides, the median inhibitory concentration （IC50） was cal- culated by MTF, and A549 and SGC-7901 cell cycles and apoptosis were observed by flow cytometry. Results The inhibitory effects of S-1 on both cells increased as the concentration went up, and signif- icantly showed concentration-dependant. The IC50 values of S-1 on A549 and SGC-7901 cells were re- spectively lower than those in 5-Fu group （78.33 μg/mL vs 241.60 μg/mL; 63.40 μg/mL vs 201. 68 μg/mL）. It was shown that S-1 can markedly result in the arrest of A549 and SGC-7901 ceils at phases G0/Gl and S, and its apoptosis rates on A549 and SGC-7901 cells were （36.64 ± 3.92） % and （38.69 ± 3.02） %, respectively, evidently higher than in blank group. Conclusion The inhibi- tory effects of S-1 on human lung cancer cell A549 and gastric cancer cell SGC-7901 in vitro are con- spicuous, notably superior to 5-Fu.