紫杉醇对SHI-1细胞株增殖抑制及诱导凋亡的作用

目的观察紫杉醇体外抑制人白血病细胞SHI-1细胞的增殖活性及诱导细胞分化和凋亡的作用，并探讨其凋亡机制。方法以不同质量浓度（5、0．5、0．05、0．005mg／L）的紫杉醇与SHI-1细胞在体外共同培养，分别作用0、12、24、36、48h，通过细胞计数、台盼蓝染色、细胞形态学、DNA含量测定、AnnexinV／PI、细胞线粒体跨膜电位（△φm）等分析细胞的凋亡。结果紫杉醇能促进SHI-1细胞的凋亡；0．05mg,／L紫杉醇处理对SHI-1细胞株增殖抑制及诱导凋亡作用最为显著。结论紫杉醇能抑制SHI-1细胞增殖活性，诱导细胞分化，促进细胞凋亡，使SHI-1细胞停留在G2／M期。

Effect of paclitaxel in inhibiting proliferation of SHI-1 and induction of apoptosis

Objective To observe the efficiency of paclitaxel in inhibiting the proliferation of human leukemia cell SHI-1 ceils and in the induction of cell differentiation, and further discuss the mechanism of apoptosis. Methods SHI-1 cells in different concentrations were cultured in vitro with paclitaxel at 0, 12h, 24h, 36h, 48 h respectively. Cellular apoptosis was then analyzed by counting cells, trypan blue staining, cell morphology, DNA content determination, Annexinv/PI and mitochon- drial transmembrane potential （Aq~m）. Results Paclitaxel facilitated the cellular apoptosis, and the concentration of 0.05 mg/L was most effective in inhibiting paclitaxel SHI-1 cellular proliferation and inducing cellular apoptosis. Conclusion Paclitaxel can inhibit the proliferation of SHI-1 cells, induce its differentiation and facilitate cellular apoptosis, making SHI-1 cells stop at the stage of G2/M.