三氧化二砷对人肝癌SMMC-7721细胞的体内外作用及可能的机制

目的观察三氧化二砷（As203）在体内外对人肝癌SMMC-7721细胞抗肿瘤作用。方法不同浓度的As203作用于SMMC-7721细胞48h，采用MTT法测定细胞的存活率；荧光显微镜观察细胞形态变化；FITC-Annexinv／P1双标记检测SMMC-7721细胞的凋亡；比色法检测Caspase．3活性变化。用As203在SMMC-7721肝癌细胞荷瘤裸鼠的瘤体内进行注射治疗，观察肿瘤生长变化，12d后处死裸鼠，摘除瘤体，称瘤重，并通过免疫组化法检测Bcl-2、Bax和Caspase-3的表达。结果As203能显著抑制SMMC-7721细胞的增殖，半数抑制率浓度为（18．17±2．10）μmol／L；显微镜下可见有典型的细胞凋亡形态学改变，As203处理组SMMC-7721细胞凋亡率与对照组相比显著增加，As203可提高SMMC-7721细胞的Caspase-3活性。As203瘤内注射肝癌细胞株SMMC-7721裸鼠移植瘤后，能显著抑制肿瘤生长，瘤重的抑制率可达52．37％，免疫组化结果显示As203能明显上调与细胞凋亡相关因子Bax和Caspase．3的表达和下调Bcl-2的表达。结论As203能抑制肝癌SMMC-7721细胞的生长，诱导SMMC-7721细胞凋亡；As203能抑制SMMC-7721细胞的体内致瘤能力。

Effect of arsenic trioxide on SMMC-7721 cells of patients with hepatoma and its potential mechanism in vivo and vitro

Objective To observe the anti-tumor effect of arsenic trioxide （As203） on SMMC-7721 cells of patients with hepatoma in vivo and vitro. Methods Methyl Thiazolyl Tetrazoli- um （MTY） method, fluorescence microscope, FITC-AnnexinV/PI double-tagging method and colori- metry were used to detect the survival rate, morphological change, apoptosis and Caspase-3 activity change of SMMC-7721 cells in different concentration of A%O3 for 48 h, respectively. AszO3 was in- jected into the transplanted tumors in nude mice with SMMC-7721 hepatoma cells to observe the growth of tumors. The mice were sacrificed after 12 d, followed by the extirpation and weighing of the tumors. Then Bcl-2, Bax and Caspase-3 expression were detected by immunohistochemistry. Results As203 could obviously inhabit the proliferation of SMMC-7721 ceils, with inhibition concentration （IC） being （18.17 ± 2. 10） μmol/L. Microscope showed typical morphological change of cell apoptosis and As203 treatment group was evidently higher than control group in apoptosis rate of SMMC-772 cells, suggesting that As203 could improve SMMC-772 cell activity. Internal injection of As203 into trans- planted tumors in nude mice with SMMC-7721 hepatocellular lines could remarkably inhabit the growth of tumors with inhabiting rate being52.37%, and immunohistochemistry also revealed that As203 could apparently up-regulate cell apoptosis-related Bax and Caspase-3 expression, and down-regulate Bcl-2 expression. Conclusion As203 can inhabit the growth and in vivo oncogenesis of hepatoma SMMC-7721 cells, and induce SMMC-7721 cell apoptosis.