外源性IL-4基因表达对K562细胞增殖及死亡的影响

目的：观察外源性ＩＬ－４对Ｋ５６２增殖及死亡的影响。方法：用逆转录病毒载体将ＩＬ－４ｃＤＮＡ导入Ｋ５６２细胞,检测其增殖反应,用流式细胞依及ＤＮＡ片段凝胶电泳检测细胞增殖周期及凋亡情况,用台盼蓝排除法计数死亡细胞。结果：高表达ＩＬ－４的Ｋ５６２细胞增殖反应明显低于表达株。亲代Ｋ５６２株;前者２过程中死亡细胞百分率明显升高且可被ＩＬ－４单克隆抗体阻断,但其自发凋亡细胞数及增殖周期均无改变。结论Ｌ：外

The effect of exogenous IL-4 gene expression on cell death of K562 cells

Objective: To observe the effect of exogenous IL 4 gene expression on cell death of K562 cells.Methods: IL 4 gene was transduced into K562 cell via retroviral vector mediated gene transfer method.Trypan blue stain,gel electrophoresis and flow cytometry were employed to determine cell death of K562 cell. Results:Proliferation of high IL 4 producing K562 clones was significantly lower than that of low IL 4 producing clones,parental K562 cells and K562 cells co cultured with recombinant IL 4.Cell death of high IL 4 producing K562 cells was enhanced dramatically and this effect could be blocked by IL 4 McAb,but its cell cycle no ever changed.There was no apoptosis determined.Conclusion: IL 4 expressed by IL 4 gene modified K562 cells could induce cell death of K562 cell by means of necrosis,which is benefitial for growth inhibition of leukemic cell.