大鼠自体动脉血脑出血动物模型的建立

目的:建立大鼠自体动脉血液脑出血模型,研究脑出血后大鼠行为学改变、脑水肿的变化规律。方法:参照Yang、Lee及Hua等方法,应用立体定向技术,用大鼠自体尾动脉不抗凝动脉血液50μl缓慢注入大鼠尾状核,制成中等量脑出血,通过动脉观察其行为学改变和脑水肿的变化规律建立稳定的脑出血动物模型。结果:对32只大鼠脑出血动物模型采用Longa评分法、肢体对称试验评分法、Berderson评分法和平衡木评分法进行评分,结果显示,大鼠脑出血后其行为学改变与对照组有显著性差异。脑出血周围组织含水量明显高于对照组,病变侧高于病变对侧脑组织,以出血后48～72h最明显。结论:大鼠自体动脉血脑出血后有一系列的行为学改变,其行为学改变能够反映脑血肿对神经功能影响的严重程度和病情的轻重,大鼠自体动脉血脑出血动物模型是比较理想的实验性脑出血模型。

Behavioral and Brain Edema after intracerebral hemorrhage in rats

Objective: To make an intracerebral hemorrhage model in rats by autologous caudate artery blood, which can be used in the field of the behavioral and brain edema research. Methods: A medium volume of hematoma was formed by stereotac-tic infusing 50 μl autologous caudate artery blood. The behavioral and brain edema following intracerebral hemorrhage were studied. Results: 32 intracerebral hemorrhage in rat model showed that there were a lot of behavioral changes and brain edema after intracerebral hemorrhage in rat. The worse behavioral changes and brain edema was at 48-72 hours after hemorrhage. Conclusions: Intracerebral hemorrhage model in rats by infusing autologous caudate artery blood was reproducible. Following the hemorrhage there were some changes on rat behavioral and brain edema.