Control of recombination within and between DNA plasmids of Saccharomyces cerevisiae |
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Authors: | Melanie J. Dobson A. Bruce Futcher Brian S. Cox |
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Affiliation: | (1) Botany School, South Parks Road, OX1 3RA Oxford, England |
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Abstract: | Summary [2 m+ and [2m°] yeast were transformed to stable leucine prototrophy with the hybrid yeast — E. coli plasmid, pJDB219. This plasmid contains the entire sequence of the endogenous 2 m yeast DNA plasmid in addition to the yeast nuclear LEU2+ gene and the Co1E1 derivative, pMB9. In the [2 m+] transformants, a new wholly yeast LEU2+ plasmid, pYX, was generated, probably by a recombination event between pJDB219 and 2 m DNA. The plamid, pYX, in the absence of 2 m DNA, was found to exist in equimolar amounts of two forms, A and B, which probably arise by intramolecular recombination across the inverted repeat sequences of the 2 m DNA portion of the plasmid. pJDB219 was found to require the presence of 2 m DNA to undergo this intramolecular recombination. The results suggest that 2, m DNA and pYX code for a gene product required in this recombination event which pJDB219 cannot produce. |
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Keywords: | Recombination Plasmids Transformation Yeast |
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