应激在急性出血坏死性胰腺炎所致肺损伤中的作用

目的:通过复制大鼠急性出血坏死性胰腺炎(AHNP)模型,探讨在AHNP的发病过程中,应激与AHNP及其所致肺损伤的关系。方法:用5%牛磺胆酸钠溶液逆行胰胆管注射制作大鼠AHNP模型。将实验动物随机分为A-C3组,A组为假手术组,B组为AHNP组,C组在制成AHNP模型后给予甲吡酮(metyrapone)以控制应激反应的程度,观察大鼠血清中皮质醇,C反应蛋白(CRP)及血清淀粉酶的含量,以及肺及胰腺的病理组织学。结果:C组动物血清中皮质醇、CRP及淀粉酶的含量明显低于对照组,肺及胰腺的损害程度,明显低于对照组(P＜0.05)。结论:应激在急性出血坏死性胰腺炎并发肺损伤的过程中起了重要作用。运用特异性抗应激药物可以明显减轻其所致的肺损伤。

Role of stress in the lung injury caused by acute hemorrhagic necrotizing pancreatitis in rats

AIM: To study the relation ship between stress and lung injury caused by acute hemorrhagic necrotizing pancreatitis(AHNP) through AHNP model.METHODS: The AHNP model was made by using 5% sodium taurocholate retrograded injection into biliopancreatic duct in SD rats. Those rats were divided into three groups randomly, from A to C, the A group undertook sham operations, the B group was made into AHNP model, and the C group was given Metyrapone. The level of corticosteroid, CRP and amylase in serum had been observed. The lung and pancreas histological examinations were also performed.RESULTS: In C group, the level of corticosteroid, CRP and amylase were much lower than those in B group. The grade of lung and pancreas injury were also lower than those in B group(P<0.05).CONCLUSION: Stress lays an important role in the lung injury caused by AHNP. Using anti-stress drugs can inhibit this course and meliorate the lung injury.