三七总皂苷对腺嘌呤致大鼠肾间质纤维化影响的实验研究

目的 观察三七总皂苷(抗血栓、抗组织缺血性损伤中药)对肾间质纤维化实验大鼠的肾脏保护作用并探讨其可能的作用机制.方法 用腺嘌呤灌胃法建立大鼠肾间质纤维化模型,将实验动物随机分为2组:实验组(三七总皂苷,35mg·kg~(-1)·d~(-1),灌服4周)和模型组(灌服等量生理盐水),并设立空白对照组.于开始实验第4,7周末,观察大鼠血清细胞间粘附分子-1(ICAM-1)、24 h尿蛋白变化和a-平滑肌肌动蛋白(a-SMA)的表达.结果 实验组、模型组的血清ICAM-1、24 h尿蛋白定量、a-SMA浓度及肾问质纤维化程度等均高于对照组(P＜0.05);但实验组低于模型组(P＜0.05).结论 三七总皂苷可能通过下调模型大鼠a-SMA及ICAM-1的表达,防治肾小管问质纤维化.

Experimental study of Panax notoginseng saponins on the renal interstitial fibrosis with adenine-induced in rats

Objective To observe the protective effects and mechanism of Panax notoginseng saponins(PNS)on the renal interstitial fibrosis rats.Methods Adenine gavage method used rat model of renal fibrosis,the experimental animals were divided into the test group(PNS,dose of 35 mg·kg~(-1)·d~(-1),fed for 4 weeks)and model group,and the establishment of the lank normal group.In the model after 4,7 weeks,the serum cell adhesion molecule-1(ICAM-1),24-hour urine protein auantitative changes and a-smooth muscle actin(a-SMA)were observed.Results In test and model group,the serum ICAM-1,24-hour urine protein,renal a-SMA in the concentration level and renal fibrosis and other indicators higher than the normal group(P＜0.05),but test group lower model group(P＜0.05).Conclusion Panax notoginseng saponins might inhibit tubuloin terstitial fibrosis in adenine nephropathy rat model by suppressing the level of ICAM-1 in serum and the expression of a-SMA in the tubulointerstitial.