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Toxicity Identification Evaluation of Lake Orta (Northern Italy) Sediments Using the Microtox System
Authors:Licia Guzzella  Cristina Bartone  Philippe Ross  Gabriele Tartari  Herbert Muntau
Institution:aWater Research Institute—CNR, via della Mornera 25, 20047, Brugherio Milan, Italy;bThe Citadel, Charleston, South Carolina;cDepartment of Biology, University of Milan, Milan, Italy;dItalian Institute of Hydrobiology—CNR, Pallanza, Novara, Italy
Abstract:Pore waters extracted by centrifugation from Lake Orta (Northern Italy) sediments were studied with a modified Toxicity Identification Evaluation (TIE) procedure using the Microtox bacterial luminescence toxicity test system. The most toxic pore water samples were from stations near a rayon factory, known as a source of copper and ammonium discharges. The TIE manipulations used were filtration, EDTA chelation, and C18 solid-phase resin adsorption. The most effective treatments to remove toxicity were the EDTA and C18, indicating that both metals and nonpolar organic compounds contribute to the observed toxicity.
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