免疫荧光法观察正畸大鼠牙周P物质神经纤维变化的研究

目的观察正畸牙齿移动不同时期大鼠牙周组织中P物质(SP)免疫阳性神经纤维的变化。方法采用冰冻切片和间接免疫荧光方法观察正畸大鼠牙周组织P物质免疫阳性神经纤维的表达变化情况。结果正常大鼠第一磨牙牙周组织中SP免疫阳性神经纤维数量稀少。正畸加力后2小时SP免疫染色反应神经纤维数量和强度开始增加,加力后1天达到最高水平,这种现象一直持续到撤力后2周,撤力后第4周SP免疫染色反应神经纤维数量和强度有所下降但仍然高于对照组水平。结论正畸牙齿移动过程中大鼠牙周组织中P物质免疫阳性神经纤维表达发生了明显的变化。提示P物质可能不仅参与早期正畸牙周组织的炎症损伤过程,而且可能参与了后期的组织修复重建过程。

Changes in the expression of substance P-containing nerve fibers in the rat periodontal ligament during and after orthodontic tooth movement by immunoflurescent method

Objective To investigate the relationship between substance P and orthodontic tooth movement.Methods The first maxillary molar was moved mesially by an orthodontic force for 2 hours,8 hours,1 days,3 days,1 week and 2 weeks,whereas the other groups were observed after removal of appliances for 1 week,2 weeks and 4 weeks.Substance P-like immunoreactivity was demonstrated by indirect immunoflurescence on frozen sections.Results The number and tensity of SP-containing nerve fibers increased after 2 hours during tooth movement;The most pronounced changes occurred in the 1 day group;After 4 weeks SP-like immunoreactivity was still higher than the control level.Conclusions The results suggest the involvement of substance P in both early stages of periodontal remodeling and later in the regenerative processes of the periodontal ligament during and after orthodontic tooth movement.